Overview

  • Product name
    Anti-PPP1A/PPP1CA antibody
    See all PPP1A/PPP1CA primary antibodies
  • Description
    Rabbit polyclonal to PPP1A/PPP1CA
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human, Zebrafish
    Predicted to work with: Mouse, Rat, Cow, Dog, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to Human PPP1A/PPP1CA aa 1-274.

  • Positive control
    • 293T and A431 whole cell lysates; PPP1A/PPP1CA siRNA; paraformaldehyde fixed HeLa cells; Human SAS xenograft tissue.
  • General notes

     This product was previously labelled as PPP1A

     

Properties

Applications

Our Abpromise guarantee covers the use of ab137512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 37 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase.
  • Sequence similarities
    Belongs to the PPP phosphatase family. PP-1 subfamily.
  • Cellular localization
    Cytoplasm. Nucleus. Nucleus > nucleoplasm. Nucleus > nucleolus. Primarily nuclear and largely excluded from the nucleolus. Highly mobile in cells and can be relocalized through interaction with targeting subunits. NOM1 plays a role in targeting this protein to the nucleolus. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha isoform serine threonine protein phosphatase PP1alpha 1 catalytic subunit antibody
    • Catalytic subunit antibody
    • EC 3.1.3.16 antibody
    • MGC15877 antibody
    • MGC1674 antibody
    • PP 1A antibody
    • PP-1A antibody
    • PP1A antibody
    • PP1A_HUMAN antibody
    • PP1alpha antibody
    • PP2C ALPHA antibody
    • PP2CA antibody
    • Ppp1ca antibody
    • Protein Phosphatase 2C Alpha Isoform antibody
    • Serine threonine protein phosphatase PP1 alpha catalytic subunit antibody
    • Serine threonine protein phosphatase PP1 alpha catalytic subunit protein phosphatase 1 antibody
    • Serine/threonine-protein phosphatase PP1-alpha catalytic subunit antibody
    see all

Images

  • All lanes : Anti-PPP1A/PPP1CA antibody (ab137512) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 37 kDa



    12% SDS PAGE
  • All lanes : Anti-PPP1A/PPP1CA antibody (ab137512) at 1/1000 dilution

    Lane 1 : U2OS cells treated with control siRNA
    Lane 2 : U2OS cells treated with PP1A/PPP1CA siRNA

    Predicted band size: 37 kDa



    Nuclear matrix protein p84 is a loading control, blotted with a p84 antibody at a 1/5000 dilution.
  • Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa cells (green) labelling PPP1A/PPP1CA with ab137512 at 1/500 dilution. Alpha tubulin filaments were labeled with an Alpha tubulin antibody (red) at 1/2500 dilution.

  • Immunohistochemical analysis of paraffin embedded Human SAS xenograft tissue labelling PPP1A/PPP1CA with ab137512 at 1/100 dilution.

References

This product has been referenced in:
  • Chen T  et al. PKCe phosphorylates MIIP and promotes colorectal cancer metastasis through inhibition of RelA deacetylation. Nat Commun 8:939 (2017). Read more (PubMed: 29038521) »
  • Carrara M  et al. Decoding the selectivity of eIF2a holophosphatases and PPP1R15A inhibitors. Nat Struct Mol Biol 24:708-716 (2017). Read more (PubMed: 28759048) »
See all 2 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Western blot
Sample
Zebrafish Tissue lysate - whole (whole embryo)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
50 µg
Specification
whole embryo
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Chi-Cheng Hsieh

Verified customer

Submitted Jul 14 2014

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