• Product name
    Anti-PPP1A/PPP1CA antibody
    See all PPP1A/PPP1CA primary antibodies
  • Description
    Rabbit polyclonal to PPP1A/PPP1CA
  • Host species
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human, Zebrafish
    Predicted to work with: Mouse, Rat, Cow, Dog, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to Human PPP1A/PPP1CA aa 1-274.

  • Positive control
    • 293T and A431 whole cell lysates; PPP1A/PPP1CA siRNA; paraformaldehyde fixed HeLa cells; Human SAS xenograft tissue.
  • General notes

     This product was previously labelled as PPP1A




Our Abpromise guarantee covers the use of ab137512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 37 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function
    Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase.
  • Sequence similarities
    Belongs to the PPP phosphatase family. PP-1 subfamily.
  • Cellular localization
    Cytoplasm. Nucleus. Nucleus > nucleoplasm. Nucleus > nucleolus. Primarily nuclear and largely excluded from the nucleolus. Highly mobile in cells and can be relocalized through interaction with targeting subunits. NOM1 plays a role in targeting this protein to the nucleolus. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha isoform serine threonine protein phosphatase PP1alpha 1 catalytic subunit antibody
    • Catalytic subunit antibody
    • EC antibody
    • MGC15877 antibody
    • MGC1674 antibody
    • PP 1A antibody
    • PP-1A antibody
    • PP1A antibody
    • PP1A_HUMAN antibody
    • PP1alpha antibody
    • PP2C ALPHA antibody
    • PP2CA antibody
    • Ppp1ca antibody
    • Protein Phosphatase 2C Alpha Isoform antibody
    • Serine threonine protein phosphatase PP1 alpha catalytic subunit antibody
    • Serine threonine protein phosphatase PP1 alpha catalytic subunit protein phosphatase 1 antibody
    • Serine/threonine-protein phosphatase PP1-alpha catalytic subunit antibody
    see all


  • All lanes : Anti-PPP1A/PPP1CA antibody (ab137512) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 37 kDa

    12% SDS PAGE
  • All lanes : Anti-PPP1A/PPP1CA antibody (ab137512) at 1/1000 dilution

    Lane 1 : U2OS cells treated with control siRNA
    Lane 2 : U2OS cells treated with PP1A/PPP1CA siRNA

    Predicted band size: 37 kDa

    Nuclear matrix protein p84 is a loading control, blotted with a p84 antibody at a 1/5000 dilution.
  • Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa cells (green) labelling PPP1A/PPP1CA with ab137512 at 1/500 dilution. Alpha tubulin filaments were labeled with an Alpha tubulin antibody (red) at 1/2500 dilution.

  • Immunohistochemical analysis of paraffin embedded Human SAS xenograft tissue labelling PPP1A/PPP1CA with ab137512 at 1/100 dilution.


This product has been referenced in:
  • Chen T  et al. PKCe phosphorylates MIIP and promotes colorectal cancer metastasis through inhibition of RelA deacetylation. Nat Commun 8:939 (2017). Read more (PubMed: 29038521) »
  • Carrara M  et al. Decoding the selectivity of eIF2a holophosphatases and PPP1R15A inhibitors. Nat Struct Mol Biol 24:708-716 (2017). Read more (PubMed: 28759048) »
See all 2 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Western blot
Zebrafish Tissue lysate - whole (whole embryo)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
50 µg
whole embryo
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Chi-Cheng Hsieh

Verified customer

Submitted Jul 14 2014

For licensing inquiries, please contact partnerships@abcam.com

Sign up