Key features and details
- Rabbit polyclonal to PPP1A/PPP1CA
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG
Product nameAnti-PPP1A/PPP1CA antibody
See all PPP1A/PPP1CA primary antibodies
DescriptionRabbit polyclonal to PPP1A/PPP1CA
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Rat, Cow, Human
Predicted to work with: Rabbit, Dog
- WB: Total rat brain homogenate.
A protein phosphatase is a phosphatase enzyme that removes a phosphate group from the phosphorylated amino acid residue of its substrate protein. Protein phosphorylation is one of the most common forms of reversible protein posttranslational modification (PTM), with up to 30% of all proteins being phosphorylated at any given time. Protein kinases (PKs) are the effectors of phosphorylation and catalyse the transfer of a γ-phosphate from ATP to specific amino acids on proteins. Several hundred PKs exist in mammals and are classified into distinct super-families. Proteins are phosphorylated predominantly on Ser, Thr and Tyr residues, which account for 79.3, 16.9 and 3.8% respectively of the phosphoproteome, at least in mammals. In contrast, protein phosphatases (PPs) are the primary effectors of dephosphorylation and can be grouped into three main classes based on sequence, structure and catalytic function. The largest class of PPs is the phosphoprotein phosphatase (PPP) family comprising PP1, PP2A, PP2B, PP4, PP5, PP6 and PP7, and the protein phosphatase Mg²?- or Mn²?-dependent (PPM) family, composed primarily of PP2C.
This product was previously labelled as PPP1A.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.08% Sodium azide
Concentration information loading...
PurityAmmonium Sulphate Precipitation
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab16446 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 38 kDa.|
|IP||Use at an assay dependent dilution.|
FunctionProtein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase.
Sequence similaritiesBelongs to the PPP phosphatase family. PP-1 subfamily.
Cellular localizationCytoplasm. Nucleus. Nucleus > nucleoplasm. Nucleus > nucleolus. Primarily nuclear and largely excluded from the nucleolus. Highly mobile in cells and can be relocalized through interaction with targeting subunits. NOM1 plays a role in targeting this protein to the nucleolus. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles.
- Information by UniProt
- Alpha isoform serine threonine protein phosphatase PP1alpha 1 catalytic subunit antibody
- Catalytic subunit antibody
- EC 126.96.36.199 antibody
ab16446 has been referenced in 1 publication.
- Freire PP et al. Obesity does not lead to imbalance between myocardial phospholamban phosphorylation and dephosphorylation. Arq Bras Cardiol 103:41-50 (2014). PubMed: 25120084