• Product name

    Anti-PPP1A/PPP1CA antibody
    See all PPP1A/PPP1CA primary antibodies
  • Description

    Rabbit polyclonal to PPP1A/PPP1CA
  • Host species

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Cow, Dog
  • Immunogen

    Synthetic peptide within Human PPP1A/PPP1CA aa 280-330. The exact sequence is proprietary. NP_002699.1
    Database link: P62136

  • Positive control

    • IHC-P: Human lung cancer tissue. WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate.
  • General notes

     This product was previously labelled as PPP1A




Our Abpromise guarantee covers the use of ab245585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase.
    • Sequence similarities

      Belongs to the PPP phosphatase family. PP-1 subfamily.
    • Cellular localization

      Cytoplasm. Nucleus. Nucleus > nucleoplasm. Nucleus > nucleolus. Primarily nuclear and largely excluded from the nucleolus. Highly mobile in cells and can be relocalized through interaction with targeting subunits. NOM1 plays a role in targeting this protein to the nucleolus. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles.
    • Information by UniProt
    • Database links

    • Alternative names

      • Alpha isoform serine threonine protein phosphatase PP1alpha 1 catalytic subunit antibody
      • Catalytic subunit antibody
      • EC antibody
      • MGC15877 antibody
      • MGC1674 antibody
      • PP 1A antibody
      • PP-1A antibody
      • PP1A antibody
      • PP1A_HUMAN antibody
      • PP1alpha antibody
      • PP2C ALPHA antibody
      • PP2CA antibody
      • Ppp1ca antibody
      • Protein Phosphatase 2C Alpha Isoform antibody
      • Serine threonine protein phosphatase PP1 alpha catalytic subunit antibody
      • Serine threonine protein phosphatase PP1 alpha catalytic subunit protein phosphatase 1 antibody
      • Serine/threonine-protein phosphatase PP1-alpha catalytic subunit antibody
      see all


    • Formalin-fixed, paraffin-embedded human lung cancer tissue stained for PPP1A/PPP1CA with ab245585 at a 1/1000 dilution in immunohistochemical analysis. DAB staining.

    • All lanes : Anti-PPP1A/PPP1CA antibody (ab245585) at 0.1 µg/ml

      Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
      Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
      Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell lineadenocarcinoma) whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Exposure time: 30 seconds

      Prepared using NETN lysis buffer.


    ab245585 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab245585.
    Please use the links above to contact us or submit feedback about this product.

    For licensing inquiries, please contact partnerships@abcam.com

    Sign up