Product nameAnti-PPP1A/PPP1CA antibody
See all PPP1A/PPP1CA primary antibodies
DescriptionRabbit polyclonal to PPP1A/PPP1CA
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Cow, Dog
Synthetic peptide within Human PPP1A/PPP1CA aa 280-330. The exact sequence is proprietary. NP_002699.1
Database link: P62136
- IHC-P: Human lung cancer tissue. WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate.
This product was previously labelled as PPP1A
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245585 was affinity purified using an epitope specific to PPP1A/PPP1CA immobilized on solid support.
Our Abpromise guarantee covers the use of ab245585 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionProtein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase.
Sequence similaritiesBelongs to the PPP phosphatase family. PP-1 subfamily.
Cellular localizationCytoplasm. Nucleus. Nucleus > nucleoplasm. Nucleus > nucleolus. Primarily nuclear and largely excluded from the nucleolus. Highly mobile in cells and can be relocalized through interaction with targeting subunits. NOM1 plays a role in targeting this protein to the nucleolus. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles.
- Information by UniProt
- Alpha isoform serine threonine protein phosphatase PP1alpha 1 catalytic subunit antibody
- Catalytic subunit antibody
- EC 188.8.131.52 antibody
Formalin-fixed, paraffin-embedded human lung cancer tissue stained for PPP1A/PPP1CA with ab245585 at a 1/1000 dilution in immunohistochemical analysis. DAB staining.
All lanes : Anti-PPP1A/PPP1CA antibody (ab245585) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell lineadenocarcinoma) whole cell lysate
Lysates/proteins at 50 µg per lane.
Exposure time: 30 seconds
Prepared using NETN lysis buffer.
ab245585 has not yet been referenced specifically in any publications.