Key features and details
- Rabbit polyclonal to PPP1A/PPP1CA
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PPP1A/PPP1CA antibody
See all PPP1A/PPP1CA primary antibodies
DescriptionRabbit polyclonal to PPP1A/PPP1CA
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Cow, Dog
Synthetic peptide within Human PPP1A/PPP1CA aa 280-330. The exact sequence is proprietary. NP_002699.1
Database link: P62136
- IHC-P: Human lung cancer tissue. WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate.
This product was previously labelled as PPP1A
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245585 was affinity purified using an epitope specific to PPP1A/PPP1CA immobilized on solid support.
Our Abpromise guarantee covers the use of ab245585 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionProtein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase.
Sequence similaritiesBelongs to the PPP phosphatase family. PP-1 subfamily.
Cellular localizationCytoplasm. Nucleus. Nucleus > nucleoplasm. Nucleus > nucleolus. Primarily nuclear and largely excluded from the nucleolus. Highly mobile in cells and can be relocalized through interaction with targeting subunits. NOM1 plays a role in targeting this protein to the nucleolus. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles.
- Information by UniProt
- Alpha isoform serine threonine protein phosphatase PP1alpha 1 catalytic subunit antibody
- Catalytic subunit antibody
- EC 18.104.22.168 antibody
Formalin-fixed, paraffin-embedded human lung cancer tissue stained for PPP1A/PPP1CA with ab245585 at a 1/1000 dilution in immunohistochemical analysis. DAB staining.
All lanes : Anti-PPP1A/PPP1CA antibody (ab245585) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell lineadenocarcinoma) whole cell lysate
Lysates/proteins at 50 µg per lane.
Exposure time: 30 seconds
Prepared using NETN lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245585 has not yet been referenced specifically in any publications.