Overview

  • Product name

    Anti-PPP1A/PPP1CA antibody
    See all PPP1A/PPP1CA primary antibodies
  • Description

    Rabbit polyclonal to PPP1A/PPP1CA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Cow, Dog
  • Immunogen

    Synthetic peptide within Human PPP1A/PPP1CA aa 280-330. The exact sequence is proprietary. NP_002699.1
    Database link: P62136

  • Positive control

    • IHC-P: Human lung cancer tissue. WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate.
  • General notes

     This product was previously labelled as PPP1A

     

Properties

Applications

Our Abpromise guarantee covers the use of ab245585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase.
    • Sequence similarities

      Belongs to the PPP phosphatase family. PP-1 subfamily.
    • Cellular localization

      Cytoplasm. Nucleus. Nucleus > nucleoplasm. Nucleus > nucleolus. Primarily nuclear and largely excluded from the nucleolus. Highly mobile in cells and can be relocalized through interaction with targeting subunits. NOM1 plays a role in targeting this protein to the nucleolus. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles.
    • Information by UniProt
    • Database links

    • Alternative names

      • Alpha isoform serine threonine protein phosphatase PP1alpha 1 catalytic subunit antibody
      • Catalytic subunit antibody
      • EC 3.1.3.16 antibody
      • MGC15877 antibody
      • MGC1674 antibody
      • PP 1A antibody
      • PP-1A antibody
      • PP1A antibody
      • PP1A_HUMAN antibody
      • PP1alpha antibody
      • PP2C ALPHA antibody
      • PP2CA antibody
      • Ppp1ca antibody
      • Protein Phosphatase 2C Alpha Isoform antibody
      • Serine threonine protein phosphatase PP1 alpha catalytic subunit antibody
      • Serine threonine protein phosphatase PP1 alpha catalytic subunit protein phosphatase 1 antibody
      • Serine/threonine-protein phosphatase PP1-alpha catalytic subunit antibody
      see all

    Images

    • Formalin-fixed, paraffin-embedded human lung cancer tissue stained for PPP1A/PPP1CA with ab245585 at a 1/1000 dilution in immunohistochemical analysis. DAB staining.

    • All lanes : Anti-PPP1A/PPP1CA antibody (ab245585) at 0.1 µg/ml

      Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
      Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
      Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell lineadenocarcinoma) whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Exposure time: 30 seconds


      Prepared using NETN lysis buffer.

    References

    ab245585 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab245585.
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