Product nameAnti-PPP1A/PPP1CA (phospho T320) antibody [EP1512Y]
See all PPP1A/PPP1CA primary antibodies
DescriptionRabbit monoclonal [EP1512Y] to PPP1A/PPP1CA (phospho T320)
SpecificityThis antibody detects PP1 alpha phosphorylated at Threonine 320.
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC
Species reactivityReacts with: Mouse, Rat, Human, Xenopus laevis
corresponding to Human PPP1A/PPP1CA.
- HeLa cell lysate and human muscle tissue.
This product was previously labelled as PPP1A
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab62334 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).|
|IHC-P||1/100 - 1/250. An antigen retrieval step is strongly recommended.|
FunctionProtein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase.
Sequence similaritiesBelongs to the PPP phosphatase family. PP-1 subfamily.
Cellular localizationCytoplasm. Nucleus. Nucleus > nucleoplasm. Nucleus > nucleolus. Primarily nuclear and largely excluded from the nucleolus. Highly mobile in cells and can be relocalized through interaction with targeting subunits. NOM1 plays a role in targeting this protein to the nucleolus. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles.
- Information by UniProt
- Alpha isoform serine threonine protein phosphatase PP1alpha 1 catalytic subunit antibody
- Catalytic subunit antibody
- EC 188.8.131.52 antibody
All lanes : Anti-PPP1A/PPP1CA (phospho T320) antibody [EP1512Y] (ab62334)
Lane 1 : HeLa cell lysate (untreated)
Lane 2 : HeLa cell lysate (treated with Calyculin A)
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP conjugate at 1/2000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
The bottom image shows beta Tubulin as a positive control.
Ab62334 (1/100-1/250) staining human PPP1A/PPP1CA in human muscle tissue by immunohistochemistry using paraffin embedded tissue.
This product has been referenced in:
- Chassé H et al. Cyclin B Translation Depends on mTOR Activity after Fertilization in Sea Urchin Embryos. PLoS One 11:e0150318 (2016). Read more (PubMed: 26962866) »
- Daldello EM et al. Control of Cdc6 accumulation by Cdk1 and MAPK is essential for completion of oocyte meiotic divisions in Xenopus. J Cell Sci 128:2482-96 (2015). Read more (PubMed: 26092930) »