Overview

  • Product name
    Anti-PPP1A/PPP1CA (phospho T320) antibody [EP1512Y]
    See all PPP1A/PPP1CA primary antibodies
  • Description
    Rabbit monoclonal [EP1512Y] to PPP1A/PPP1CA (phospho T320)
  • Host species
    Rabbit
  • Specificity
    This antibody detects PP1 alpha phosphorylated at Threonine 320.
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Xenopus laevis
  • Immunogen

    corresponding to Human PPP1A/PPP1CA.

  • Positive control
    • HeLa cell lysate and human muscle tissue.
  • General notes

     This product was previously labelled as PPP1A

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab62334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
IP 1/40.
IHC-P 1/100 - 1/250. An antigen retrieval step is strongly recommended.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function
      Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase.
    • Sequence similarities
      Belongs to the PPP phosphatase family. PP-1 subfamily.
    • Cellular localization
      Cytoplasm. Nucleus. Nucleus > nucleoplasm. Nucleus > nucleolus. Primarily nuclear and largely excluded from the nucleolus. Highly mobile in cells and can be relocalized through interaction with targeting subunits. NOM1 plays a role in targeting this protein to the nucleolus. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha isoform serine threonine protein phosphatase PP1alpha 1 catalytic subunit antibody
      • Catalytic subunit antibody
      • EC 3.1.3.16 antibody
      • MGC15877 antibody
      • MGC1674 antibody
      • PP 1A antibody
      • PP-1A antibody
      • PP1A antibody
      • PP1A_HUMAN antibody
      • PP1alpha antibody
      • PP2C ALPHA antibody
      • PP2CA antibody
      • Ppp1ca antibody
      • Protein Phosphatase 2C Alpha Isoform antibody
      • Serine threonine protein phosphatase PP1 alpha catalytic subunit antibody
      • Serine threonine protein phosphatase PP1 alpha catalytic subunit protein phosphatase 1 antibody
      • Serine/threonine-protein phosphatase PP1-alpha catalytic subunit antibody
      see all

    Images

    • All lanes : Anti-PPP1A/PPP1CA (phospho T320) antibody [EP1512Y] (ab62334)

      Lane 1 : HeLa cell lysate (untreated)
      Lane 2 : HeLa cell lysate (treated with Calyculin A)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugate at 1/2000 dilution

      Predicted band size: 38 kDa
      Observed band size: 38 kDa



      The bottom image shows beta Tubulin as a positive control.
    • Ab62334 (1/100-1/250) staining human PPP1A/PPP1CA in human muscle tissue by immunohistochemistry using paraffin embedded tissue.

    References

    This product has been referenced in:
    • Chassé H  et al. Cyclin B Translation Depends on mTOR Activity after Fertilization in Sea Urchin Embryos. PLoS One 11:e0150318 (2016). Read more (PubMed: 26962866) »
    • Daldello EM  et al. Control of Cdc6 accumulation by Cdk1 and MAPK is essential for completion of oocyte meiotic divisions in Xenopus. J Cell Sci 128:2482-96 (2015). Read more (PubMed: 26092930) »
    See all 6 Publications for this product

    Customer reviews and Q&As

    Question
    Answer

    I am very pleased to hear you would like to test ab62334 in rabbit. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forrabbitand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

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