Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1511Y] to PPP1CA + PPP1CB (HRP)
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
Product nameAnti-PPP1CA + PPP1CB antibody [EP1511Y] (HRP)
See all PPP1CA + PPP1CB primary antibodies
DescriptionRabbit monoclonal [EP1511Y] to PPP1CA + PPP1CB (HRP)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human PPP1CA + PPP1CB aa 200-300. The exact sequence is proprietary.
Database link: P62140
- WB: HeLa whole cell and Mouse Brain and Rat Brain tissue lysates.
This product was previously labelled as Protein Phosphatase 1 beta, PPP1CA + 1CB
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab211372 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa).|
Cellular localizationCytoplasm. Nucleus. Nucleus > nucleoplasm. Nucleus > nucleolus.
- PP-1A antibody
- PP-1B antibody
- PP1A antibody
All lanes : Anti-PPP1CA + PPP1CB antibody [EP1511Y] (HRP) (ab211372) at 1/5000 dilution
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 :
HeLa whole cell lysate (ab150035)
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab211372 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab211372 has not yet been referenced specifically in any publications.