Overview

  • Product name

    Anti-PPP1CA + PPP1CB antibody [EP1511Y] (HRP)
    See all PPP1CA + PPP1CB primary antibodies
  • Description

    Rabbit monoclonal [EP1511Y] to PPP1CA + PPP1CB (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PPP1CA + PPP1CB aa 200-300. The exact sequence is proprietary.
    Database link: P62140

  • Positive control

    • WB: HeLa whole cell and Mouse Brain and Rat Brain tissue lysates.
  • General notes

     

     

     This product was previously labelled as Protein Phosphatase 1 beta, PPP1CA + 1CB

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab211372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa).

Target

Images

  • All lanes : Anti-PPP1CA + PPP1CB antibody [EP1511Y] (HRP) (ab211372) at 1/5000 dilution

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : HeLa whole cell lysate (ab150035)

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 37 kDa
    Observed band size: 36 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab211372 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab211372 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab211372.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up