Key features and details
- Rabbit polyclonal to PPP1CB
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PPP1CB antibody
See all PPP1CB primary antibodies
DescriptionRabbit polyclonal to PPP1CB
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Xenopus tropicalis
Recombinant fragment corresponding to Human PPP1CB aa 1-274.
- 293T, A431, H1299, HeLaS3, HepG2, Molt-4 and Raji whole cell lysate. Mouse brain whole cell lysate. HeLa cells.
General notesKeep as concentrated solution.
This product was previously labelled as Protein Phosphatase 1 beta, PPP1CA + 1CB
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154600 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 37 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).|
|ICC/IF||1/100 - 1/1000.|
FunctionProtein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208).
Sequence similaritiesBelongs to the PPP phosphatase family. PP-1 subfamily.
Cellular localizationCytoplasm. Nucleus. Nucleus, nucleoplasm. Nucleus, nucleolus. Highly mobile in cells and can be relocalized through interaction with targeting subunits. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles.
- Information by UniProt
- MGC3672 antibody
- PP 1B antibody
- PP-1B antibody
All lanes : Anti-PPP1CB antibody (ab154600) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lane 4 : HeLa S3 whole cell lysate
Lane 5 : Hep G2 whole cell lysate
Lane 6 : MOLT4 whole cell lysate
Lane 7 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 37 kDa
7.5% SDS PAGE
Anti-PPP1CB antibody (ab154600) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 37 kDa
10% SDS PAGE
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling PPP1CB with ab154600 at 1/500 dilution. Lower panel is co-stained with Hoechst 33342.
Immunohistochemical analysis of paraffin-embedded A549 xenograft labelling PPP1CB with ab154600 at 1/100 dilution.
ab154600 has been referenced in 1 publication.
- Gershoni-Emek N et al. Proteomic Analysis of Dynein-Interacting Proteins in Amyotrophic Lateral Sclerosis Synaptosomes Reveals Alterations in the RNA-Binding Protein Staufen1. Mol Cell Proteomics 15:506-22 (2016). ICC/IF . PubMed: 26598648