Overview

  • Product name

  • Description

    Rabbit polyclonal to PPP1CB
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Chicken, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human PPP1CB (internal sequence). The exact sequence is proprietary.
    Database link: P62140

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts; Mouse midbrain tissue extract; Rat brain tissue extract.
  • General notes

     This product was previously labelled as Protein Phosphatase 1 beta, PPP1CA + 1CB

     

Properties

Applications

Our Abpromise guarantee covers the use of ab227620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 37 kDa.

Target

  • Function

    Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208).
  • Sequence similarities

    Belongs to the PPP phosphatase family. PP-1 subfamily.
  • Cellular localization

    Cytoplasm. Nucleus. Nucleus, nucleoplasm. Nucleus, nucleolus. Highly mobile in cells and can be relocalized through interaction with targeting subunits. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles.
  • Information by UniProt
  • Database links

  • Alternative names

    • MGC3672 antibody
    • PP 1B antibody
    • PP-1B antibody
    • PP1B antibody
    • PP1B_HUMAN antibody
    • PP1beta antibody
    • PPP1CB antibody
    • PPP1CD antibody
    • Protein phosphatase 1 beta antibody
    • Protein phosphatase 1 catalytic subunit beta isoform antibody
    • Protein phosphatase 1 delta antibody
    • Protein phosphatase 1, catalytic subunit, beta isozyme antibody
    • Protein phosphatase 1, catalytic subunit, delta isoform antibody
    • Serine threonine protein phosphatase PP1 beta catalytic subunit antibody
    • Serine/threonine-protein phosphatase PP1-beta catalytic subunit antibody
    see all

Images

  • All lanes : Anti-PPP1CB antibody (ab227620) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 37 kDa



    10% SDS-PAGE gel.

  • All lanes : Anti-PPP1CB antibody (ab227620) at 1/1000 dilution

    Lane 1 : Mouse midbrain tissue extract
    Lane 2 : Rat brain tissue extract

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 37 kDa



    10% SDS-PAGE gel.

References

ab227620 has not yet been referenced specifically in any publications.

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