Overview

  • Product name

    Anti-PPP1CB antibody [EP1804Y]
    See all PPP1CB primary antibodies
  • Description

    Rabbit monoclonal [EP1804Y] to PPP1CB
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, Flow Cyt, IHC-P, ICC, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human PPP1CB aa 300 to the C-terminus.

  • Positive control

    • Jurkat whole cell lysate (ab7899) Human muscle tissue
  • General notes

     This product was previously labelled as Protein Phosphatase 1 beta, PPP1CA + 1CB.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab53315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).

For unpurified use at 1/10000 - 1/20000.

IP 1/100.
Flow Cyt 1/20 - 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For unpurified use at 1/250.

See IHC antigen retrieval protocols.

ICC Use at an assay dependent concentration.
ICC/IF 1/250.

Target

  • Function

    Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208).
  • Sequence similarities

    Belongs to the PPP phosphatase family. PP-1 subfamily.
  • Cellular localization

    Cytoplasm. Nucleus. Nucleus, nucleoplasm. Nucleus, nucleolus. Highly mobile in cells and can be relocalized through interaction with targeting subunits. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles.
  • Information by UniProt
  • Database links

  • Alternative names

    • MGC3672 antibody
    • PP 1B antibody
    • PP-1B antibody
    • PP1B antibody
    • PP1B_HUMAN antibody
    • PP1beta antibody
    • PPP1CB antibody
    • PPP1CD antibody
    • Protein phosphatase 1 beta antibody
    • Protein phosphatase 1 catalytic subunit beta isoform antibody
    • Protein phosphatase 1 delta antibody
    • Protein phosphatase 1, catalytic subunit, beta isozyme antibody
    • Protein phosphatase 1, catalytic subunit, delta isoform antibody
    • Serine threonine protein phosphatase PP1 beta catalytic subunit antibody
    • Serine/threonine-protein phosphatase PP1-beta catalytic subunit antibody
    see all

Images

  • All lanes : Anti-PPP1CB antibody [EP1804Y] (ab53315) at 1/50000 dilution (purified)

    Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
    Lane 2 : Mouse brain lysates
    Lane 3 : Rat brain lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 37 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cervical carcinoma tissue sections labeling Protein phosphatase 1 beta with purified ab53315 at 1:400 dilution (1 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH 9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Protein phosphatase 1 beta with purified ab53315 at 1:40 dilution (10 ug/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - 90% methanol. Unlabeled control - Rabbit monoclonal IgG (Black). Cell without incubation with primary antibody and secondary antibody (Blue).

  • ab53315 (purified) at 1:100 dilution (2ug) immunoprecipitating Protein phosphatase 1 beta in Human fetal brain lysate.

    Lane 1 (input): Human fetal brain lysate 10ug

    Lane 2 (+): ab53315 & Human fetal brain lysate

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab53315 in Human fetal brain lysate

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.

    Blocking and diluting buffer: 5% NFDM/TBST.

  • Unpurified ab53315 staining Protein Phosphatase 1 beta antibody in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000.

    DAPI was used as a nuclear counterstain and the negative control was PBS only.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat kidney tissue sections labeling Protein phosphatase 1 beta with purified ab53315 at 1:400 dilution (1 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH 9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue sections labeling Protein phosphatase 1 beta with purified ab53315 at 1:400 dilution (1 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH 9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Anti-PPP1CB antibody [EP1804Y] (ab53315) at 1/20000 dilution (unpurified) + Jurkat cells at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

    Predicted band size: 37 kDa

  • Immunocytochemistry/ Immunofluorescence analysis of MCF-7 (Human breast adenocarcinoma epithelial cell) cells labeling Protein phosphatase 1 beta with Purified ab53315 at 1:250 dilution (1.6μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemical analysis of paraffin-embedded sections of human cervix carcinoma labelling Protein Phosphatase 1 beta with unpurified ab53315 at 1:250 dilution. Prediluted ImmunoHistoprobe (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody.

    Counter stain = Hematoxylin. Heat mediated antigen retrieval using EDTA buffer at pH 9 was performed.

  • Immunohistochemical analysis of paraffin-embedded sections of mouse cardiac muscle labelling Protein Phosphatase 1 beta with unpurified ab53315 at 1:250 dilution. Prediluted ImmunoHistoprobe (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody.

    Counter stain = Hematoxylin. Heat mediated antigen retrieval using EDTA buffer at pH 9 was performed.

  • Immunohistochemical analysis of paraffin-embedded sections of rat colon labelling Protein Phosphatase 1 beta with unpurified ab53315 at 1:250 dilution. Prediluted ImmunoHistoprobe (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody.

    Counter stain = Hematoxylin. Heat mediated antigen retrieval using EDTA buffer at pH 9 was performed.

  • Flow Cytometry analysis of HeLa cells labelling Protein Phosphatase 1 beta with unpurified ab53315 at 1:20 dilution. Goat anti rabbit IgG (H&L) (FITC) at a dilution of 1:150 used as the secondary antibody. Isotype control = Rabbit monoclonal IgG. Fixative=2% paraformaldehyde.

References

This product has been referenced in:

  • Vuono EA  et al. Interaction of Structural Glycoprotein E2 of Classical Swine Fever Virus with Protein Phosphatase 1 Catalytic Subunit Beta (PPP1CB). Viruses 11:N/A (2019). Read more (PubMed: 30934875) »
  • Carey BD  et al. Protein Phosphatase 1a Interacts with Venezuelan Equine Encephalitis Virus Capsid Protein and Regulates Viral Replication through Modulation of Capsid Phosphorylation. J Virol 92:N/A (2018). Read more (PubMed: 29769351) »
See all 19 Publications for this product

Customer reviews and Q&As

1-2 of 2 Q&A

Question
Answer

Thank you for your phone call. The immunogen sequence for ab53315 is C-terminal. I hope this helps, please let me know if you need any additional information or assistance.

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Question
Answer

Thank you for your enquiry. The epitope is right on the C-terminus, AA 310-327.

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