Recombinant
RabMAb

Recombinant Anti-PPP1R11 antibody [EPR11965] (ab171960)

Overview

  • Product name

    Anti-PPP1R11 antibody [EPR11965]
    See all PPP1R11 primary antibodies
  • Description

    Rabbit monoclonal [EPR11965] to PPP1R11
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PPP1R11 aa 100 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: O60927

  • Positive control

    • Human testis, HeLa, fetal brain, NIH: OVCAR-3 and MDA-MB-435 lysates. Permeabilized HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11965
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab171960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    Images

    • All lanes : Anti-PPP1R11 antibody [EPR11965] (ab171960) at 1/1000 dilution

      Lane 1 : Human testis lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Fetal brain lysate
      Lane 4 : NIH: OVCAR-3 lysate
      Lane 5 : MDA-MB-435 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 14 kDa

    • Flow cytometric analysis of permeabilized HeLa cells labeling PPP1R11 with ab171960 at 1/10 dilution (red) or a Rabbit IgG (negative) (green).

    • Western blot analysis on immunoprecipitation pellet from Human testis lysate (1) labeling PPP1R11 with ab171960 at 1/10 dilution or 1X PBS (2) negative control. HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.

    References

    ab171960 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (MLE-12)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    MLE-12
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

    Abcam user community

    Verified customer

    Submitted Feb 17 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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