Overview

  • Product name

  • Description

    Rabbit polyclonal to PPP1R3B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human PPP1R3B (internal sequence). The exact sequence is proprietary.
    Database link: Q86XI6

  • Positive control

    • WB: MCF7 and MDA-MB-231 whole cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab229586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 33 kDa.

Target

  • Function

    Acts as a glycogen-targeting subunit for phosphatase PP1. Facilitates interaction of the PP1 with enzymes of the glycogen metabolism and regulates its activity. Suppresses the rate at which PP1 dephosphorylates (inactivates) glycogen phosphorylase and enhances the rate at which it activates glycogen synthase and therefore limits glycogen breakdown. Its activity is inhibited by PYGL, resulting in inhibition of the glycogen synthase and glycogen phosphorylase phosphatase activities of PP1. Dramatically increases basal and insulin-stimulated glycogen synthesis upon overexpression in hepatocytes.
  • Tissue specificity

    Highly expressed in liver. Expressed moderately in skeletal muscle and myotubes (at protein level). Expressed predominantly in heart and skeletal muscle. Expressed moderately in liver. Expressed weakly in placenta, lung and kidney.
  • Sequence similarities

    Contains 1 CBM21 (carbohydrate binding type-21) domain.
  • Domain

    The N-terminal region is required for binding to PP1, the central region is required for binding to glycogen and the C-terminal region is required for binding to PYGL.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ14005 antibody
    • FLJ34675 antibody
    • GL antibody
    • Hepatic glycogen targeting protein phosphatase 1 regulatory subunit GL antibody
    • Hepatic glycogen targeting subunit G(L) antibody
    • Hepatic glycogen-targeting protein phosphatase 1 regulatory subunit GL antibody
    • PP1 subunit R4 antibody
    • Ppp1r3b antibody
    • PPP1R4 antibody
    • PPR3B_HUMAN antibody
    • Protein phosphatase 1 regulatory (inhibitor) subunit 3B antibody
    • Protein phosphatase 1 regulatory subunit 3B antibody
    • Protein phosphatase 1 regulatory subunit 4 antibody
    • Protein phosphatase 1 subunit 4 antibody
    • Protein phosphatase 1 subunit GL antibody
    • PTG antibody
    • R4 antibody
    see all

Images

  • All lanes : Anti-PPP1R3B antibody (ab229586) at 1/1000 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell extract
    Lane 2 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 33 kDa



    12% SDS-PAGE gel.

References

ab229586 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229586.
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