Key features and details
- Rabbit polyclonal to PPP1R3B
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-PPP1R3B antibody
See all PPP1R3B primary antibodies
DescriptionRabbit polyclonal to PPP1R3B
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow
Recombinant full length protein corresponding to Human PPP1R3B aa 1-285.
MMAVDIEYRYNCMAPSLRQERFAFKISPKPSKPLRPCIQLSSKNEASGMV APAVQEKKVKKRVSFADNQGLALTMVKVFSEFDDPLDMPFNITELLDNIV SLTTAESESFVLDFSQPSADYLDFRNRLQADHVCLENCVLKDKAIAGTVK VQNLAFEKTVKIRMTFDTWKSYTDFPCQYVKDTYAGSDRDTFSFDISLPE KIQSYERMEFAVYYECNGQTYWDSNRGKNYRIIRAELKSTQGMTKPHSGP DLGISFDQFGSPRCSYGLFPEWPSYLGYEKLGPYY
Database link: Q86XI6
- WB: Rat heart and mouse liver tissue lysate. IHC-P: Human skeletal muscle and liver tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab235049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000.|
|IHC-P||1/20 - 1/200.|
FunctionActs as a glycogen-targeting subunit for phosphatase PP1. Facilitates interaction of the PP1 with enzymes of the glycogen metabolism and regulates its activity. Suppresses the rate at which PP1 dephosphorylates (inactivates) glycogen phosphorylase and enhances the rate at which it activates glycogen synthase and therefore limits glycogen breakdown. Its activity is inhibited by PYGL, resulting in inhibition of the glycogen synthase and glycogen phosphorylase phosphatase activities of PP1. Dramatically increases basal and insulin-stimulated glycogen synthesis upon overexpression in hepatocytes.
Tissue specificityHighly expressed in liver. Expressed moderately in skeletal muscle and myotubes (at protein level). Expressed predominantly in heart and skeletal muscle. Expressed moderately in liver. Expressed weakly in placenta, lung and kidney.
Sequence similaritiesContains 1 CBM21 (carbohydrate binding type-21) domain.
DomainThe N-terminal region is required for binding to PP1, the central region is required for binding to glycogen and the C-terminal region is required for binding to PYGL.
- Information by UniProt
- FLJ14005 antibody
- FLJ34675 antibody
- GL antibody
Anti-PPP1R3B antibody (ab235049) at 1/500 dilution + Mouse liver tissue lysate
Goat polyclonal to rabbit at 1/10000 dilution
Paraffin-embedded human liver tissue stained for PPP1R3B using ab235049 at 1/100 dilution in immunohistochemical analysis.
Anti-PPP1R3B antibody (ab235049) at 1/500 dilution + Rat heart tissue lysate
Goat polyclonal to rabbit at 1/50000 dilution
Paraffin-embedded human skeletal muscle tissue stained for PPP1R3B using ab235049 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235049 has not yet been referenced specifically in any publications.