Product nameAnti-PPP2CB antibody
See all PPP2CB primary antibodies
DescriptionRabbit polyclonal to PPP2CB
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Drosophila melanogaster, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
Synthetic peptide corresponding to Human PPP2CB. Synthetic peptide from a region between residue 260 and the C-terminus (residue 309) of human PPP2CB
- Whole cell lysate from Hela, NIH3T3 and 293T cells.
This product was previously labelled as PP2A beta
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72343 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 28, 36, 190 kDa (predicted molecular weight: 36 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionPP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase.
Sequence similaritiesBelongs to the PPP phosphatase family. PP-1 subfamily.
modificationsReversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. Demethylated by PME1 (in vitro).
Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
Cellular localizationCytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles.
- Information by UniProt
- PP2A beta antibody
- PP2A-beta antibody
- PP2AB_HUMAN antibody
All lanes : Anti-PPP2CB antibody (ab72343) at 0.1 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
Developed using the ECL technique.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Additional bands at: 190 kDa, 28 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Immunoprecipitation/ Western Blot of PPP2CB.
Lane 1: ab72343 at 3µg/mg whole cell lysate. Lane 2: Control IgG.
ab72343 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 30 seconds.
ab72343 has been referenced in 1 publication.
- Joseph BK et al. Inhibition of AMP Kinase by the Protein Phosphatase 2A Heterotrimer, PP2APpp2r2d. J Biol Chem 290:10588-98 (2015). PubMed: 25694423