Product nameAnti-PPP2CB antibody [EPR11786(B)]
See all PPP2CB primary antibodies
DescriptionRabbit monoclonal [EPR11786(B)] to PPP2CB
Tested applicationsSuitable for: IP, ICC/IF, Flow Cyt, WBmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human PPP2CB.
Database link: P62714
- 293T, HeLa, Human fetal brain, Human fetal heart and NIH 3T3 lysates; 293T and NIH3T3 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab168371 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/10 - 1/100.|
|ICC/IF||1/50 - 1/100.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/10000 - 1/50000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).|
FunctionPP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase.
Sequence similaritiesBelongs to the PPP phosphatase family. PP-1 subfamily.
modificationsReversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. Demethylated by PME1 (in vitro).
Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
Cellular localizationCytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles.
- Information by UniProt
- PP2A beta antibody
- PP2A-beta antibody
- PP2AB_HUMAN antibody
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: PPP2CB knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: A431 whole cell lysate (20 µg)
ab168371 was shown to specifically react with PPP2CB in wild-type HAP1 cells. No band was observed when PPP2CB knockout samples were examined. Wild-type and PPP2CB knockout samples were subjected to SDS-PAGE. Ab168371 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/10,000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
Detection of PPP2CB by Western Blot of Immunprecipitate.
293T cell lysate immunoprecipitated using ab168371 at 1/10 dilution.
For WB detection ab168371 at 1/10000 dilution was used.
HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Flow Cytometric analysis of permeabilized NIH 3T3 cells labeling PPP2CB with ab168371 at 1/100 dilution (red) or a rabbit IgG (negative) (green).
Immunofluorescent analysis of 293T cells labeling PPP2CB with ab168371 at 1/50 dilution.
All lanes : Anti-PPP2CB antibody [EPR11786(B)] (ab168371) at 1/10000 dilution
Lane 1 : 293T lysate
Lane 2 : HeLa lysate
Lane 3 : Human fetal brain lysate
Lane 4 : Human fetal heart lysate
Lane 5 : NIH3T3 lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 36 kDa
ab168371 has not yet been referenced specifically in any publications.