Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-PPP2CB antibody [EPR11786(B)] - BSA and Azide free (ab249465)

Overview

  • Product name
    Anti-PPP2CB antibody [EPR11786(B)] - BSA and Azide free
    See all PPP2CB primary antibodies
  • Description
    Rabbit monoclonal [EPR11786(B)] to PPP2CB - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, Flow Cyt, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • General notes

    ab249465 is a PBS-only buffer format of ab168371. Please refer to ab168371 for recommended dilutions, protocols, and image data.

     

     This product was previously labelled as PP2A beta

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity
    Affinity purified
  • Clonality
    Monoclonal
  • Clone number
    EPR11786(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab249465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
Flow Cyt Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase.
    • Sequence similarities
      Belongs to the PPP phosphatase family. PP-1 subfamily.
    • Post-translational
      modifications
      Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. Demethylated by PME1 (in vitro).
      Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
    • Cellular localization
      Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles.
    • Information by UniProt
    • Database links
    • Alternative names
      • PP2A beta antibody
      • PP2A-beta antibody
      • PP2AB_HUMAN antibody
      • PP2Abeta antibody
      • PP2CB antibody
      • Ppp2cb antibody
      • Protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform antibody
      • Protein phosphatase 2 catalytic subunit beta isozyme antibody
      • Protein phosphatase 2, catalytic subunit, beta isoform antibody
      • Protein phosphatase 2A catalytic subunit beta isoform antibody
      • Protein phosphatase type 2A catalytic subunit antibody
      • Serine/threonine protein phosphatase 2A catalytic subunit beta antibody
      • Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform antibody
      see all

    References

    ab249465 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab249465.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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