Recombinant Anti-PPP2R1A antibody [6G3] - BSA and Azide free (ab255976)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [6G3] to PPP2R1A - BSA and Azide free
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PPP2R1A antibody [6G3] - BSA and Azide free
See all PPP2R1A primary antibodies -
Description
Rat monoclonal [6G3] to PPP2R1A - BSA and Azide free -
Host species
Rat -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein corresponding to Human PPP2R1A.
Database link: P30153 -
Positive control
- WB: HeLa, Jurkat ,Ramos, HepG2, C6 and RAW 264.7 whole cell lysate. ICC/IF: HeLa, RAW 264.7 and C6 cells.
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General notes
ab255976 is the carrier-free version of ab24736.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
6G3 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab255976 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.785 µg/ml. Predicted molecular weight: 65 kDa.
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ICC/IF |
Use a concentration of 7.85 µg/ml.
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Notes |
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WB
Use a concentration of 0.785 µg/ml. Predicted molecular weight: 65 kDa. |
ICC/IF
Use a concentration of 7.85 µg/ml. |
Target
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Function
The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis. -
Sequence similarities
Belongs to the phosphatase 2A regulatory subunit A family.
Contains 15 HEAT repeats. -
Domain
Each HEAT repeat appears to consist of two alpha helices joined by a hydrophilic region, the intrarepeat loop. The repeat units may be arranged laterally to form a rod-like structure. -
Cellular localization
Cytoplasm. Chromosome > centromere. Centromeric localization requires the presence of BUB1. - Information by UniProt
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Database links
- Entrez Gene: 5518 Human
- Entrez Gene: 51792 Mouse
- Entrez Gene: 117281 Rat
- Omim: 605983 Human
- SwissProt: P30153 Human
- SwissProt: Q76MZ3 Mouse
- Unigene: 467192 Human
- Unigene: 294138 Mouse
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Alternative names
- 2AAA_HUMAN antibody
- 6330556D22Rik antibody
- Alpha isoform of regulatory subunit A, protein phosphatase antibody
see all
Images
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling PPP2R1A with ab24736 at 7.85µg/ml, followed by Goat Anti-Rat IgG (Alexa Fluor® 488) (ab150157) secondary antibody at 1/1000 dilution (green). Confocal image showing mainly showing cytoplasmic staining on HeLa cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab24736 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution.
-ve control 2: ab179504 at 1/200 dilution, followed by ab150157 AlexaFluor®488 Goat anti-rat secondary at 1/1000 dilution.This image was produced using the same antibody clone but in a different formulation ab24736, PBS, sodium azide, glycerol and BSA.
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All lanes : Anti-PPP2R1A antibody [6G3] (ab24736) at 0.785 µg/ml
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 3 : Ramos (Human Burkitt’s lymphoma B lymphocyte), whole cell lysate
Lane 4 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lane 5 : C6 (rat glial tumor glial cell), whole cell lysate
Lane 6 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution
Predicted band size: 65 kDa
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
This image was produced using the same antibody clone but in a different formulation ab24736, PBS, sodium azide, glycerol and BSA.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C6 (rat glial tumor glial cell) cells labeling PPP2R1A with ab24736 at 7.85µg/ml, followed by Goat Anti-Rat IgG (Alexa Fluor® 488) (ab150157) secondary antibody at 1/1000 dilution (green). Confocal image showing mainly showing cytoplasmic staining on C6 cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab24736 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution.
-ve control 2: ab179504 at 1/200 dilution, followed by ab150157 AlexaFluor®488 Goat anti-rat secondary at 1/1000 dilution.This image was produced using the same antibody clone but in a different formulation ab24736, PBS, sodium azide, glycerol and BSA.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling PPP2R1A with ab24736 at 7.85µg/ml, followed by Goat Anti-Rat IgG (Alexa Fluor® 488) (ab150157) secondary antibody at 1/1000 dilution (green). Confocal image showing mainly showing cytoplasmic staining on RAW 264.7 cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab24736 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution.
-ve control 2: ab179504 at 1/200 dilution, followed by ab150157 AlexaFluor®488 Goat anti-rat secondary at 1/1000 dilution.This image was produced using the same antibody clone but in a different formulation ab24736, PBS, sodium azide, glycerol and BSA.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab255976 has been referenced in 1 publication.
- Kremmer E et al. Separation of PP2A core enzyme and holoenzyme with monoclonal antibodies against the regulatory A subunit: abundant expression of both forms in cells. Mol Cell Biol 17:1692-701 (1997). PubMed: 9032296