Validated using a knockout cell line
Recombinant
RabMAb

Anti-PPP2R1B antibody [EPR10158] (ab154815)

Overview

  • Product name
    Anti-PPP2R1B antibody [EPR10158]
    See all PPP2R1B primary antibodies
  • Description
    Rabbit monoclonal [EPR10158] to PPP2R1B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PPP2R1B (internal sequence). The exact sequence is proprietary.

  • Positive control
    • WB: HeLa, HAP1, Caco 2 and Jurkat cell lysates. ICC/IF: Caco-2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR10158
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 66 kDa.
Flow Cyt Use at an assay dependent concentration.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit.
    • Sequence similarities
      Belongs to the phosphatase 2A regulatory subunit A family.
      Contains 15 HEAT repeats.
    • Domain
      Each HEAT repeat appears to consist of two alpha helices joined by a hydrophilic region, the intrarepeat loop. The repeat units may be arranged laterally to form a rod-like structure.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2AAB_HUMAN antibody
      • beta isoform of regulatory subunit A, protein phosphatase 2 antibody
      • PP2A A beta antibody
      • PP2A subunit A isoform PR65-beta antibody
      • PP2A subunit A isoform R1-beta antibody
      • PP2A, subunit A, PR65 beta isoform antibody
      • PP2A, subunit A, R1 beta isoform antibody
      • PPP2R1B antibody
      • PPP4R1 antibody
      • PR65B antibody
      • protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), beta isoform antibody
      • protein phosphatase 2 (formerly 2A), regulatory subunit A, beta isoform antibody
      • Protein phosphatase 2, regulatory subunit A, beta antibody
      • Protein phosphatase 2, structural/regulatory subunit A, beta antibody
      • protein phosphatase 2, structural/regulatory subunit A, beta isoform antibody
      • serine/threonine protein phosphatase 2A 65 kDa regulatory subunit A, beta isoform antibody
      • serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, beta isoform antibody
      • Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform antibody
      see all

    Images

    • Flow Cytometry analysis of Caco-2 (human colorectal adenocarcinoma ) cells labeling PPP2R1B with unpurified ab154815 at 1:40 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1:2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: PPP2R1B knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Caco2 cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab154815 observed at 67 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab154815 was shown to specifically react with PPP2R1B when PPP2R1B knockout samples were used. Wild-type and PPP2R1B knockout samples were subjected to SDS-PAGE. ab154815 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-PPP2R1B antibody [EPR10158] (ab154815) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Caco 2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 66 kDa

    • Immunofluorescence analysis of Caco 2 cells, labeling PPP2R1B using ab154815 at 1/50 dilution.

    References

    This product has been referenced in:
    • Lahlali T  et al. Netrin-1 Protects Hepatocytes Against Cell Death Through Sustained Translation During the Unfolded Protein Response. Cell Mol Gastroenterol Hepatol 2:281-301.e9 (2016). Read more (PubMed: 28174720) »

    See 1 Publication for this product

    Customer reviews and Q&As

    We have not tested 7288-1 or 7301-1 for cross reactivity with PP2AA alpha, however, we have only observed one band in our in-house testing with 7288-1 and we have detected only 2 isoforms of PP2AA beta with 7301-1. It is possible they could react, base...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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