Product nameAnti-PPP2R1B antibody [EPR10159]
See all PPP2R1B primary antibodies
DescriptionRabbit monoclonal [EPR10159] to PPP2R1B
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide, corresponding to residues in Human PPP2R1B (UniProt: P30154).
- Caco2 and Jurkat cell lysates.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab154833 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 66 kDa.|
FunctionThe PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit.
Sequence similaritiesBelongs to the phosphatase 2A regulatory subunit A family.
Contains 15 HEAT repeats.
DomainEach HEAT repeat appears to consist of two alpha helices joined by a hydrophilic region, the intrarepeat loop. The repeat units may be arranged laterally to form a rod-like structure.
- Information by UniProt
- 2AAB_HUMAN antibody
- beta isoform of regulatory subunit A, protein phosphatase 2 antibody
- PP2A A beta antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: PPP2R1B knockout HAP1 cell lysate (20 µg)
Lane 3: Jurkat cell lysate (20 µg)
Lane 4: Caco2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab154833 observed at 67 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab154833 was shown to recognize PPP2R1B when PPP2R1B knockout samples were used, along with additional cross-reactive bands. Wild-type and ProteinX knockout samples were subjected to SDS-PAGE. ab154833 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-PPP2R1B antibody [EPR10159] (ab154833) at 1/1000 dilution
Lane 1 : Caco2 cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 66 kDa
ab154833 has not yet been referenced specifically in any publications.