Synthetic peptide, corresponding to residues in Human PPP2R1B (UniProt: P30154).
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab154833 in the following tested applications.
|WB||1/1000 - 1/10000. Predicted molecular weight: 66 kDa.|
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: PPP2R1B knockout HAP1 cell lysate (20 µg)
Lane 3: Jurkat cell lysate (20 µg)
Lane 4: Caco2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab154833 observed at 67 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab154833 was shown to recognize PPP2R1B when PPP2R1B knockout samples were used, along with additional cross-reactive bands. Wild-type and ProteinX knockout samples were subjected to SDS-PAGE. ab154833 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
ab154833 has not yet been referenced specifically in any publications.