Validated using a knockout cell line
Recombinant
RabMAb

Anti-PPP2R1B antibody [EPR10159] (ab154833)

Overview

  • Product name
    Anti-PPP2R1B antibody [EPR10159]
    See all PPP2R1B primary antibodies
  • Description
    Rabbit monoclonal [EPR10159] to PPP2R1B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human PPP2R1B (UniProt: P30154).

  • Positive control
    • Caco2 and Jurkat cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR10159
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 66 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function
      The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit.
    • Sequence similarities
      Belongs to the phosphatase 2A regulatory subunit A family.
      Contains 15 HEAT repeats.
    • Domain
      Each HEAT repeat appears to consist of two alpha helices joined by a hydrophilic region, the intrarepeat loop. The repeat units may be arranged laterally to form a rod-like structure.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2AAB_HUMAN antibody
      • beta isoform of regulatory subunit A, protein phosphatase 2 antibody
      • PP2A A beta antibody
      • PP2A subunit A isoform PR65-beta antibody
      • PP2A subunit A isoform R1-beta antibody
      • PP2A, subunit A, PR65 beta isoform antibody
      • PP2A, subunit A, R1 beta isoform antibody
      • PPP2R1B antibody
      • PPP4R1 antibody
      • PR65B antibody
      • protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), beta isoform antibody
      • protein phosphatase 2 (formerly 2A), regulatory subunit A, beta isoform antibody
      • Protein phosphatase 2, regulatory subunit A, beta antibody
      • Protein phosphatase 2, structural/regulatory subunit A, beta antibody
      • protein phosphatase 2, structural/regulatory subunit A, beta isoform antibody
      • serine/threonine protein phosphatase 2A 65 kDa regulatory subunit A, beta isoform antibody
      • serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, beta isoform antibody
      • Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: PPP2R1B knockout HAP1 cell lysate (20 µg)
      Lane 3: Jurkat cell lysate (20 µg)
      Lane 4: Caco2 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab154833 observed at 67 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab154833 was shown to recognize PPP2R1B when PPP2R1B knockout samples were used, along with additional cross-reactive bands. Wild-type and ProteinX knockout samples were subjected to SDS-PAGE. ab154833 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-PPP2R1B antibody [EPR10159] (ab154833) at 1/1000 dilution

      Lane 1 : Caco2 cell lysate
      Lane 2 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 66 kDa

    References

    ab154833 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    We have not tested 7288-1 or 7301-1 for cross reactivity with PP2AA alpha, however, we have only observed one band in our in-house testing with 7288-1 and we have detected only 2 isoforms of PP2AA beta with 7301-1. It is possible they could react, base...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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