The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC: Use at a concentration of 2 - 10 µg/ml.
IHC-Fr: Use at an assay dependent dilution (PMID 19762425).
IP: Use at an assay dependent dilution (PMID 19762425).
WB: Use at a concentration of 5 - 10 µg/ml. Detects a band of approximately 55 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
Expressed in all tissues examined.
Belongs to the phosphatase 2A regulatory subunit B family. Contains 7 WD repeats.
O'Shaughnessy RF et al. Akt-dependent Pp2a activity is required for epidermal barrier formation during late embryonic development. Development136:3423-31 (2009).
IP, WB, IF, IHC-Fr
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