Product nameAnti-PPP2R2B antibody
See all PPP2R2B primary antibodies
DescriptionRabbit polyclonal to PPP2R2B
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Cow, Pig, Orangutan
Synthetic peptide within Human PPP2R2B aa 25-75. The exact sequence is proprietary. NP_004567.1
Database link: Q00005
- WB: HeLa, HEK-293T and TCMK whole cell lysate. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264160 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 52 kDa.|
FunctionThe B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. Within the PP2A holoenzyme complex, isoform 2 is required to promote proapoptotic activity (By similarity). Isoform 2 regulates neuronal survival through the mitochondrial fission and fusion balance.
Involvement in diseaseDefects in PPP2R2B are the cause of spinocerebellar ataxia type 12 (SCA12) [MIM:604326]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA12 is an autosomal dominant cerebellar ataxia (ADCA).
Sequence similaritiesBelongs to the phosphatase 2A regulatory subunit B family.
Contains 7 WD repeats.
DomainThe N-terminal 26 residues of isoform 2 constitute a cryptic mitochondrial matrix import signal with critical basic and hydrophobic residues, that is necessary and sufficient for targeting the PP2A holoenzyme to the outer mitochondrial membrane (OMM) and does not affect holoenzyme formation or catalytic activity.
The last WD repeat of isoform 2 constitutes a mitochondrial stop-transfer domain that confers resistance to the unfolding step process required for import and therefore prevents PPP2R2B matrix translocation and signal sequence cleavage.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Membrane and Cytoplasm. Mitochondrion. Mitochondrion outer membrane. Under basal conditions, localizes to both cytosolic and mitochondrial compartments. Relocalizes from the cytosolic to the mitochondrial compartment during apoptosis. Its targeting to the outer mitochondrial membrane (OMM) involves an association with import receptors of the TOM complex and is required to promote proapoptotic activity.
- Information by UniProt
- 2ABB_HUMAN antibody
- B55 beta antibody
- Beta isoform of regulatory subunit B55 protein phosphatase 2 antibody
All lanes : Anti-PPP2R2B antibody (ab264160) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : TCMK (Mouse kidney epithelial cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
All lanes : Goat anti-Rabbit Light Chain HRP Conjugate
Predicted band size: 52 kDa
Exposure time: 3 minutes
PPP2R2B was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264160 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab264160 at 0.4 µg/ml.
Lane 1: ab264160 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 10 secs.
Cells prepared using NETN lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264160 has not yet been referenced specifically in any publications.