Anti-PPP2R3A antibody (ab126195)
Key features and details
- Rabbit polyclonal to PPP2R3A
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PPP2R3A antibody
See all PPP2R3A primary antibodies -
Description
Rabbit polyclonal to PPP2R3A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 921-1129 of Human PPP2R3A (Q06190).
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Positive control
- NT2D1, U-87 MG and MCF7 whole cell lysates; Human Colon carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126195 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 130 kDa.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 130 kDa. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. -
Tissue specificity
Expressed in heart, brain, placenta, lung, muscle and kidney. -
Sequence similarities
Contains 2 EF-hand domains. - Information by UniProt
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Database links
- Entrez Gene: 5523 Human
- Omim: 604944 Human
- SwissProt: Q06190 Human
- Unigene: 518155 Human
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Alternative names
- AI118493 antibody
- DNA for thyroid hormone receptor binding site (258bp) antibody
- P2R3A_HUMAN antibody
see all
Images
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All lanes : Anti-PPP2R3A antibody (ab126195) at 1/1000 dilution
Lane 1 : NT2D1 whole cell lysate
Lane 2 : U-87 MG whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 130 kDa
5% SDS PAGE -
ab126195, at 1/250 dilution, staining PPP2R3A in Paraffin-embedded Human Colon carcinoma tissue by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab126195 has been referenced in 2 publications.
- Song G et al. Deletion of Pr72 causes cardiac developmental defects in Zebrafish. PLoS One 13:e0206883 (2018). PubMed: 30481179
- Yang J et al. Deletion of Pr130 Interrupts Cardiac Development in Zebrafish. Int J Mol Sci 17:N/A (2016). WB ; Zebrafish . PubMed: 27845735