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Rabbit polyclonal to PPP2R3B
Predicted to work with:
Rhesus monkey, Gorilla, Orangutan
Synthetic peptide from a region between residue 350 and 400 of human PPP2R3B, NP_037371.2.
Whole cell lysate from Hela and 293T cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 2-5µg/mg of lysate.
WB: 1/2000 - 1/10000. Detects bands of approximately 65 and 200 kDa (predicted molecular weight: 65 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
Contains 1 EF-hand domain.
Information by UniProt
NY REN 8 antibody
NY REN 8 antigen antibody
Western blot - Anti-PPP2R3B antibody (ab72027)
All lanes : Anti-PPP2R3B antibody (ab72027) at 0.1 µg/ml Lane 1 : Whole cell lysate from Hela cells at 50 µg Lane 2 : Whole cell lysate from Hela cells at 15 µg Lane 3 : Whole cell lysate from Hela cells at 5 µg Lane 4 : Whole cell lysate from 293T cells at 50 µg Predicted band size: 65 kDa Observed band size: 65 kDa Additional bands at: 200 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - Anti-PPP2R3B antibody (ab72027)
Immunoprecipitation/ Western Blot of PPP2R3B.
Lane 1: ab72027 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
For western blot detection step ab72027 was used at 1 µg/ml.
Chemiluminescence with an exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"