Key features and details
- Rabbit polyclonal to PPP2R3B
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PPP2R3B antibody
See all PPP2R3B primary antibodies
DescriptionRabbit polyclonal to PPP2R3B
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rhesus monkey, Gorilla, Orangutan
Synthetic peptide from a region between residue 350 and 400 of human PPP2R3B, NP_037371.2.
- Whole cell lysate from Hela and 293T cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72027 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000. Detects bands of approximately 65 and 200 kDa (predicted molecular weight: 65 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionThe B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
Sequence similaritiesContains 1 EF-hand domain.
- Information by UniProt
- NY REN 8 antibody
- NY REN 8 antigen antibody
- P2R3B_HUMAN antibody
All lanes : Anti-PPP2R3B antibody (ab72027) at 0.1 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 65 kDa
Observed band size: 65 kDa
Additional bands at: 200 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation/ Western Blot of PPP2R3B.
Lane 1: ab72027 at 3µg/mg whole cell lysate. Lane 2: Control IgG.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. For western blot detection step ab72027 was used at 1 µg/ml.
Chemiluminescence with an exposure time of 30 seconds.
ab72027 has not yet been referenced specifically in any publications.