Key features and details
- Rabbit polyclonal to PPP2R4
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PPP2R4 antibody
See all PPP2R4 primary antibodies
DescriptionRabbit polyclonal to PPP2R4
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit
Recombinant fragment within Human PPP2R4 (internal sequence). The exact sequence is proprietary.
Database link: Q15257
- ICC/IF: HeLa cells. WB: A549 whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227243 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 41 kDa.|
FunctionPPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Acts as a regulatory subunit for serine/threonine-protein phosphatase 2A (PP2A) modulating its activity or substrate specificity, probably by inducing a conformational change in the catalytic subunit, a proposed direct target of the PPIase. Can reactivate inactive phosphatase PP2A-phosphatase methylesterase complexes (PP2A(i)) in presence of ATP and Mg(2+) (By similarity). Reversibly stimulates the variable phosphotyrosyl phosphatase activity of PP2A core heterodimer PP2A(D) in presence of ATP and Mg(2+) (in vitro). The phosphotyrosyl phosphatase activity is dependent of an ATPase activty of the PP2A(D):PPP2R4 complex. Is involved in apoptosis; the function appears to be independent from PP2A.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the PTPA-type PPIase family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- subunit B'' antibody
- KIAA0044 antibody
- MGC2184 antibody
Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PPP2R4 (green) using ab227243 at 1/500 dilution in ICC/IF.
Alpha-tubulin filaments were labeled red.
Anti-PPP2R4 antibody (ab227243) at 1/1000 dilution + A549 (human lung carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 41 kDa
10% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227243 has not yet been referenced specifically in any publications.