Key features and details
- Rabbit polyclonal to PPP2R4
- Suitable for: WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
Product nameAnti-PPP2R4 antibody
See all PPP2R4 primary antibodies
DescriptionRabbit polyclonal to PPP2R4
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Rat
Predicted to work with: Rabbit, Cow, Human
Recombinant full length protein corresponding to Rat PPP2R4 aa 1-323. (Expressed in E.coli).
MAEGERQPPPDSSEETPSATPTFIIPKKEIHTVPDMGKWKRSQAYADYIG FILTLNEGVKGKKLTFDYKVSEAIEKLVALLDTLDRWIDETPPVDQPSRF GNKAYRTWYAKLDQEAENLVATVIPTNLAAAVPEVAVYLKEAVGNSTRID YGTGHEAAFAAFLCCLCKIGVLRVDDQVAIVFKVFDRYLEVMRKLQKTYR MEPAGSQGVWGLDDFQFLPFIWGSSQLIDHPHLEPRHFVDEKAVSENHKD YMFLQCILFITEMKTGPFAEHSNQLWNISAVPSWSKVNQGLIRMYKAECL EKFPVIQHFKFGSLLPIHPVTSG
Database link: B2RYQ2
- IHC-P: Rat testis and stomach tissues. WB: Rat serum; Rat testis and brain lysates; Recombinant rat PPP2R4 protein.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab231129 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231129 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 37 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionPPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Acts as a regulatory subunit for serine/threonine-protein phosphatase 2A (PP2A) modulating its activity or substrate specificity, probably by inducing a conformational change in the catalytic subunit, a proposed direct target of the PPIase. Can reactivate inactive phosphatase PP2A-phosphatase methylesterase complexes (PP2A(i)) in presence of ATP and Mg(2+) (By similarity). Reversibly stimulates the variable phosphotyrosyl phosphatase activity of PP2A core heterodimer PP2A(D) in presence of ATP and Mg(2+) (in vitro). The phosphotyrosyl phosphatase activity is dependent of an ATPase activty of the PP2A(D):PPP2R4 complex. Is involved in apoptosis; the function appears to be independent from PP2A.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the PTPA-type PPIase family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- subunit B'' antibody
- KIAA0044 antibody
- MGC2184 antibody
Paraffin-embedded rat testis tissue stained for PPP2R4 using ab231129 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded rat stomach tissue stained for PPP2R4 using ab231129 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Recombinant rat PPP2R4 protein
HRP-Linked Guinea pig Anti-Rabbit antibody at 1/2000 dilution
Predicted band size: 37 kDa
All lanes : Anti-PPP2R4 antibody (ab231129) at 1 µg/ml
Lane 1 : Rat serum
Lane 2 : Rat testis lysate
Lane 3 : Rat brain lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit antibody at 1/2000 dilution
Predicted band size: 37 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231129 has not yet been referenced specifically in any publications.