Anti-PPP2R5A antibody (ab89621)
Key features and details
- Mouse polyclonal to PPP2R5A
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PPP2R5A antibody
See all PPP2R5A primary antibodies -
Description
Mouse polyclonal to PPP2R5A -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human PPP2R5A. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- Human placenta tissue lysate Jurkat cell lysate Transfected 293T cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89621 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 56 kDa.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 56 kDa. |
Target
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Function
The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. -
Tissue specificity
Widely expressed with the highest expression in heart and skeletal muscle. -
Sequence similarities
Belongs to the phosphatase 2A regulatory subunit B56 family. -
Post-translational
modificationsPhosphorylated on serine residues. -
Cellular localization
Cytoplasm. Nucleus. Chromosome > centromere. From mitotic prophase to metaphase, localizes at the inner centromere between a pair of sister kinetochores. Decreased expression at the onset of anaphase. - Information by UniProt
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Database links
- Entrez Gene: 5525 Human
- Omim: 601643 Human
- SwissProt: Q15172 Human
- Unigene: 497684 Human
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Alternative names
- 2A5A_HUMAN antibody
- B56A antibody
- MGC131915 antibody
see all
Images
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Anti-PPP2R5A antibody (ab89621) at 1 µg/ml + Human placenta tissue lysate at 50 µg
Predicted band size: 56 kDa -
Anti-PPP2R5A antibody (ab89621) at 1 µg/ml + Jurkat cell lysate at 50 µg
Predicted band size: 56 kDa -
All lanes : Anti-PPP2R5A antibody (ab89621) at 1 µg/ml
Lane 1 : PPP2R5A transfected 293T cell lysate
Lane 2 : Untransfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 56 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (4)
ab89621 has been referenced in 4 publications.
- Vangala D et al. Secondary resistance to anti-EGFR therapy by transcriptional reprogramming in patient-derived colorectal cancer models. Genome Med 13:116 (2021). PubMed: 34271981
- Qiu Z et al. A Genome-Wide Pooled shRNA Screen Identifies PPP2R2A as a Predictive Biomarker for the Response to ATR and CHK1 Inhibitors. Cancer Res 80:3305-3318 (2020). PubMed: 32522823
- Greenwood EJ et al. Temporal proteomic analysis of HIV infection reveals remodelling of the host phosphoproteome by lentiviral Vif variants. Elife 5:N/A (2016). PubMed: 27690223
- Porter IM et al. Bod1 regulates protein phosphatase 2A at mitotic kinetochores. Nat Commun 4:2677 (2013). WB ; Human . PubMed: 24157919