Product namePPP2R5A overexpression 293T lysate (whole cell)
ab94164 is a 293T cell transfected lysate in which Human PPP2R5A has been transiently over-expressed using a pCMV-PPP2R5A plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundFunction: The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. Tissue specificity: Widely expressed with the highest expression in heart and skeletal muscle. Similarity: Belongs to the phosphatase 2A regulatory subunit B56 family. PTM: Phosphorylated on serine residues.
Our Abpromise guarantee covers the use of ab94164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94164 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-PPP2R5A antibody (ab89621) at 1/500 dilution
Lane 1 :
PPP2R5A overexpression 293T lysate (whole cell) (ab94164)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94164 has not yet been referenced specifically in any publications.