Anti-PPP2R5B antibody (ab97295)
Key features and details
- Rabbit polyclonal to PPP2R5B
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PPP2R5B antibody
See all PPP2R5B primary antibodies -
Description
Rabbit polyclonal to PPP2R5B -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human PPP2R5B aa 434-497. The exact sequence is proprietary.
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Positive control
- A431 cell lysate
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab97295 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 57 kDa. |
Target
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Function
The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. The phosphorylated form mediates the interaction between AKT1 and PP2A phosphatase leading to dephosphorylation of AKT1 on the 'Thr-308' and 'Ser-373' residues. -
Tissue specificity
Highest expression in brain. -
Sequence similarities
Belongs to the phosphatase 2A regulatory subunit B56 family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5526 Human
- NCBI: 5453952 Human
- Omim: 601644 Human
- SwissProt: Q15173 Human
- Unigene: 75199 Human
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Alternative names
- 2A5B_HUMAN antibody
- B56B antibody
- Beta isoform of regulatory subunit B56 protein phosphatase 2A antibody
see all
Images
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Western blot - Anti-PPP2R5B antibody (ab97295)Anti-PPP2R5B antibody (ab97295) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 57 kDa
7.5% SDS PAGE
References (0)
ab97295 has not yet been referenced specifically in any publications.
