Key features and details
- Rabbit polyclonal to PPP2R5C
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PPP2R5C antibody
See all PPP2R5C primary antibodies
DescriptionRabbit polyclonal to PPP2R5C
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human PPP2R5C aa 440-490. The exact sequence is proprietary. NP_002710.2
Database link: Q13362
- IP: HEK-293T whole cell lysate.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab264337 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
Use at 2-10 µg/mg of lysate.
FunctionThe B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. The PP2A-PPP2R5C holoenzyme may specifically dephosphorylate and activate TP53 and play a role in DNA damage-induced inhibition of cell proliferation. PP2A-PPP2R5C may also regulate the ERK signaling pathway through ERK dephosphorylation.
Tissue specificityHighest levels in heart, skeletal muscle and brain. Lower levels in pancreas, kidney, lung and placenta. Very low levels in liver.
Sequence similaritiesBelongs to the phosphatase 2A regulatory subunit B56 family.
modificationsIsoform Gamma-3 is phosphorylated on serine residues. Isoform Gamma-1 phosphorylation by ERK2 is IER3-dependent and inhibits ERK dephosphorylation by PP2A-PPP2R5C.
Cellular localizationNucleus. Chromosome > centromere.
- Information by UniProt
- 2A5G_HUMAN antibody
- B' alpha regulatory subunit antibody
- B56G antibody
PPP2R5C was immunoprecipitated from HEK-293T whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264337 at 6 μg/mg lysate. Western blot was performed on the immunoprecipitates using an alternative antibody.
Lane 1: ab264337 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Exposure time: 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264337 has not yet been referenced specifically in any publications.