Anti-PPP2R5C antibody (ab264337)
Key features and details
- Rabbit polyclonal to PPP2R5C
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PPP2R5C antibody
See all PPP2R5C primary antibodies -
Description
Rabbit polyclonal to PPP2R5C -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human PPP2R5C aa 440-490. The exact sequence is proprietary. NP_002710.2
Database link: Q13362 -
Positive control
- IP: HEK-293T whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab264337 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. The PP2A-PPP2R5C holoenzyme may specifically dephosphorylate and activate TP53 and play a role in DNA damage-induced inhibition of cell proliferation. PP2A-PPP2R5C may also regulate the ERK signaling pathway through ERK dephosphorylation. -
Tissue specificity
Highest levels in heart, skeletal muscle and brain. Lower levels in pancreas, kidney, lung and placenta. Very low levels in liver. -
Sequence similarities
Belongs to the phosphatase 2A regulatory subunit B56 family. -
Post-translational
modificationsIsoform Gamma-3 is phosphorylated on serine residues. Isoform Gamma-1 phosphorylation by ERK2 is IER3-dependent and inhibits ERK dephosphorylation by PP2A-PPP2R5C. -
Cellular localization
Nucleus. Chromosome > centromere. - Information by UniProt
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Database links
- Entrez Gene: 5527 Human
- Omim: 601645 Human
- SwissProt: Q13362 Human
- Unigene: 368264 Human
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Alternative names
- 2A5G_HUMAN antibody
- B' alpha regulatory subunit antibody
- B56G antibody
see all
Images
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PPP2R5C was immunoprecipitated from HEK-293T whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264337 at 6 μg/mg lysate. Western blot was performed on the immunoprecipitates using an alternative antibody.
Lane 1: ab264337 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Exposure time: 30 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab264337 has not yet been referenced specifically in any publications.