Product nameAnti-PPP2R5C antibody
See all PPP2R5C primary antibodies
DescriptionRabbit polyclonal to PPP2R5C
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human PPP2R5C aa 490-524. The exact sequence is proprietary. NP_002710.2
Database link: Q13362
- IHC-P: Human breast carcinoma tissue; IP: HeLa whole cell lysate; WB: HeLa, HEK-293T and Jurkat whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264338 in the following tested applications.
|WB||1/1000 - 1/5000.|
|IP||Use at 2-10 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionThe B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. The PP2A-PPP2R5C holoenzyme may specifically dephosphorylate and activate TP53 and play a role in DNA damage-induced inhibition of cell proliferation. PP2A-PPP2R5C may also regulate the ERK signaling pathway through ERK dephosphorylation.
Tissue specificityHighest levels in heart, skeletal muscle and brain. Lower levels in pancreas, kidney, lung and placenta. Very low levels in liver.
Sequence similaritiesBelongs to the phosphatase 2A regulatory subunit B56 family.
modificationsIsoform Gamma-3 is phosphorylated on serine residues. Isoform Gamma-1 phosphorylation by ERK2 is IER3-dependent and inhibits ERK dephosphorylation by PP2A-PPP2R5C.
Cellular localizationNucleus. Chromosome > centromere.
- Information by UniProt
- 2A5G_HUMAN antibody
- B' alpha regulatory subunit antibody
- B56G antibody
All lanes : Anti-PPP2R5C antibody (ab264338) at 0.04 µg/ml
Lane 1 : HEK-293T whole cell lysate at 50 µg
Lane 2 : HEK-293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 3 minutes
PPP2R5C was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264338 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264338 at 1 µg/ml.
Lane 1: ab264338 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 30 secs.
Immunohistochemical analysis of FFPE human breast carcinoma tissue section labeling PPP2R5C with ab264338 at 1/1000 dilution. Hematoxylin was used as counterstain. Detection: DAB.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
ab264338 has not yet been referenced specifically in any publications.