Key features and details
- Rabbit polyclonal to PPP2R5D
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PPP2R5D antibody
See all PPP2R5D primary antibodies
DescriptionRabbit polyclonal to PPP2R5D
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Opossum, Orangutan, Elephant
Synthetic peptide corresponding to a region between residues 1 and 50 of human PPP2R5D (NP_006236.1).
- Whole cell lysate from HeLa cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72303 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 67 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionThe B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
Tissue specificityIsoform Delta-2 is widely expressed. Isoform Delta-1 is highly expressed in brain.
Sequence similaritiesBelongs to the phosphatase 2A regulatory subunit B56 family.
Cellular localizationCytoplasm. Nucleus. Nuclear in interphase, nuclear during mitosis.
- Information by UniProt
- 2A5D_HUMAN antibody
- B'delta antibody
- B56D antibody
All lanes : Anti-PPP2R5D antibody (ab72303) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Developed using the ECL technique.
Predicted band size: 67 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
Detection of Human PPP2R5D by Immunoprecipitation in whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72303 at 3 µg/mg lysate for IP and at 0.1 µg/ml for subsequent Western blot detection.
Detection: Chemiluminescence with an exposure time of 10 seconds. seconds
ab72303 has not yet been referenced specifically in any publications.