Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
100 µg
Specification
HeLa
Gel Running Conditions
Reduced Denaturing (12% gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit HRP-conjugated ab.
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 70 kDa Non-specific: 50 kDa
Abcam user community
Verified customer
Submitted Sep 28 2012