Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling PPP2R5D using ab188323 at 1/100 dilution. A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin.
Western blot - Anti-PPP2R5D antibody [EPR15617] (ab188323)
All lanes : Anti-PPP2R5D antibody [EPR15617] (ab188323) at 1/100000 dilution
Lane 1 : C6 cell lysate Lane 2 : HepG2 cell lysate Lane 3 : 293T cell lysate Lane 4 : HeLa cell lysate Lane 5 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 70 kDa Observed band size: 70 kDa
Based on the sequence analysis, ab188323 recognizes 3 isoforms with the predicted MWs of 58KDa, 66KDa and 70KDa, respectively.
Immunofluorescence analysis of HepG2 cells labeling PPP2R5D using ab188323 at 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% Paraformaldehyde. Counterstain: DAPI.
Flow cytometry analysis of HeLa cells labeling PPP2R5D using ab188323 at 1/40 dilution (pink). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG (green).