Overview

  • Product name

    Anti-PPP2R5E antibody [EPR17146]
    See all PPP2R5E primary antibodies
  • Description

    Rabbit monoclonal [EPR17146] to PPP2R5E
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PPP2R5E aa 1-100. The exact sequence is proprietary.
    Database link: Q16537

  • Positive control

    • WB: 293, C6 and NIH/3T3 cell lysates. Human fetal brain, Mouse brain, Rat brain and Human pancreas lysates. ICC/IF: C6 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab198290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
ICC/IF 1/500.

Target

  • Function

    The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
  • Sequence similarities

    Belongs to the phosphatase 2A regulatory subunit B56 family.
  • Post-translational
    modifications

    Phosphorylated on serine residues.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2A5E_HUMAN antibody
    • Epsilon isoform of regulatory subunit B56 protein phosphatase 2A antibody
    • PP2A B subunit B' epsilon antibody
    • PP2A B subunit B' epsilon isoform antibody
    • PP2A B subunit B56 epsilon antibody
    • PP2A B subunit B56 epsilon isoform antibody
    • PP2A B subunit isoform B''-epsilon antibody
    • PP2A B subunit isoform B'-epsilon antibody
    • PP2A B subunit isoform B56-epsilon antibody
    • PP2A B subunit isoform PR61-epsilon antibody
    • PP2A B subunit isoform R5-epsilon antibody
    • PP2A B subunit PR61 epsilon antibody
    • PP2A B subunit PR61 epsilon isoform antibody
    • PP2A B subunit R5 epsilon antibody
    • PP2A B subunit R5 epsilon isoform antibody
    • PPP2R5E antibody
    • Protein phosphatase 2 regulatory subunit B (B56) epsilon isoform antibody
    • Protein phosphatase 2 regulatory subunit B' epsilon antibody
    • Protein phosphatase 2 regulatory subunit B' epsilon isoform antibody
    • Regulatory subunit B of protein phosphatase 2 epsilon isoform antibody
    • Serine/threonine protein phosphatase 2A 56 kDa regulatory subunit epsilon antibody
    • Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform antibody
    see all

Images

  • Anti-PPP2R5E antibody [EPR17146] (ab198290) at 1/1000 dilution + 293 (Human epithelial cells from embryonic kidney) cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 55 kDa
    Observed band size: 55 kDa



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Anti-PPP2R5E antibody [EPR17146] (ab198290) at 1/1000 dilution + Human fetal brain lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 55 kDa
    Observed band size: 55 kDa



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Anti-PPP2R5E antibody [EPR17146] (ab198290) at 1/1000 dilution + Human pancreas lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 55 kDa
    Observed band size: 55 kDa



    Blocking/dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-PPP2R5E antibody [EPR17146] (ab198290) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate
    Lane 3 : C6 (Rat glial tumor cell) lysate
    Lane 4 : NIH/3T3 (Mouse embryo fibroblast cells) lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 55 kDa
    Observed band size: 55 kDa



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized C6 (Rat glial tumor) cells labeling PPP2R5E with ab198290 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on C6 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:-
    -ve control 1 - ab198290 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

     

References

ab198290 has not yet been referenced specifically in any publications.

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