Recombinant
RabMAb

Recombinant Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500)

Overview

  • Product name
    Anti-PPP2R5E antibody [EPR17147] - C-terminal
    See all PPP2R5E primary antibodies
  • Description
    Rabbit monoclonal [EPR17147] to PPP2R5E - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, ICC/IF, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PPP2R5E aa 450 to the C-terminus. The exact sequence is proprietary.
    Database link: Q16537

  • Positive control
    • WB: HeLa, 293 and Human skeletal muscle lysates. IHC: Human cervix carcinoma and bladder transitional cell carcinoma tissues. ICC/IF/IP: HeLa and MCF7 cells. FC: HeLa cell lysate
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab198500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IP 1/75.
ICC/IF 1/500.
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt 1/2500.

Target

  • Function
    The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
  • Sequence similarities
    Belongs to the phosphatase 2A regulatory subunit B56 family.
  • Post-translational
    modifications
    Phosphorylated on serine residues.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2A5E_HUMAN antibody
    • Epsilon isoform of regulatory subunit B56 protein phosphatase 2A antibody
    • PP2A B subunit B' epsilon antibody
    • PP2A B subunit B' epsilon isoform antibody
    • PP2A B subunit B56 epsilon antibody
    • PP2A B subunit B56 epsilon isoform antibody
    • PP2A B subunit isoform B''-epsilon antibody
    • PP2A B subunit isoform B'-epsilon antibody
    • PP2A B subunit isoform B56-epsilon antibody
    • PP2A B subunit isoform PR61-epsilon antibody
    • PP2A B subunit isoform R5-epsilon antibody
    • PP2A B subunit PR61 epsilon antibody
    • PP2A B subunit PR61 epsilon isoform antibody
    • PP2A B subunit R5 epsilon antibody
    • PP2A B subunit R5 epsilon isoform antibody
    • PPP2R5E antibody
    • Protein phosphatase 2 regulatory subunit B (B56) epsilon isoform antibody
    • Protein phosphatase 2 regulatory subunit B' epsilon antibody
    • Protein phosphatase 2 regulatory subunit B' epsilon isoform antibody
    • Regulatory subunit B of protein phosphatase 2 epsilon isoform antibody
    • Serine/threonine protein phosphatase 2A 56 kDa regulatory subunit epsilon antibody
    • Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform antibody
    see all

Images

  • All lanes : Anti-PPP2R5E antibody [EPR17147] - C-terminal (ab198500) at 1/5000 dilution

    Lane 1 : HeLa cell lysate at 10 µg
    Lane 2 : 293 cell lysate at 10 µg
    Lane 3 : Human skeletal muscle lysate at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 55 kDa
    Observed band size: 55 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling PPP2R5E with ab198500 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human cervix carcinoma tissue isobserved. Counter-stained with Hematoxylin.

    Negative control: Using PBS instead of primary antibody.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (Human breast carcinoma) cells labeling PPP2R5E with ab198500 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on MCF7 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows;
    1. ab198500 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

     

  • Flow cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PPP2R5E (red) with purified ab198500 at a 1/2500 dilution (1ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

  • PPP2R5E was immunoprecipitated from 1mg of HeLa (Human cervix adenocarcinoma) whole cell extract with ab198500 at 1/175 dilution. Western blot was performed from the immunoprecipitate using ab198500 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: HeLa whole cell extract 10µg (Input).

    Lane 2: HeLa whole cell extract

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab198500 in HeLa whole cell extract.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human cervix adenocarcinoma) cells labeling PPP2R5E with ab198500 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HeLa cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows;
    1. ab198500 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

     

  • Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling PPP2R5E with ab198500 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human transitional cell carcinoma of bladder tissue isobserved. Counter-stained with Hematoxylin.

    Negative control: Using PBS instead of primary antibody.

References

ab198500 has not yet been referenced specifically in any publications.

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