Overview

  • Product name

  • Description

    Rabbit polyclonal to PPP3CB
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human PPP3CB aa 1-228. The exact sequence is proprietary.
    Sequence:

    MAAPEPARAA PPPPPPPPPP PGADRVVKAV PFPPTHRLTS EEVFDLDGIP RVDVLKNHLV KEGRVDEEIA LRIINEGAAI LRREKTMIEV EAPITVCGDI HGQFFDLMKL FEVGGSPANT RYLFLGDYVD RGYFSIECVL YLWVLKILYP STLFLLRGNH ECRHLTEYFT FKQECKIKYS ERVYEACMEA FDSLPLAALL NQQFLCVHGG LSPEIHTLDD IRRLDRFK


    Database link: P16298

  • Positive control

    • Human brain cortex, Molt-4 and mouse brain lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab191374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
IHC-P Use a concentration of 7.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).

Target

  • Function

    Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.
  • Sequence similarities

    Belongs to the PPP phosphatase family. PP-2B subfamily.
  • Information by UniProt
  • Database links

  • Alternative names

    • Calcineurin A beta antibody
    • Calcineurin A2 antibody
    • Calcineurin B, formerly antibody
    • Calmodulin dependent calcineurin A subunit beta isoform antibody
    • Calmodulin-dependent calcineurin A subunit beta isoform antibody
    • CALNA 2 antibody
    • CALNA2 antibody
    • CALNB antibody
    • CAM PRP catalytic subunit antibody
    • CAM-PRP catalytic subunit antibody
    • CNA2 antibody
    • CnAbeta antibody
    • PP2BB_HUMAN antibody
    • PP2Bbeta antibody
    • PPP3CB antibody
    • Protein phosphatase 2B, catalytic subunit, beta isoform, formerly antibody
    • Protein phosphatase 3 (formerly 2B) catalytic subunit beta isoform antibody
    • Protein phosphatase 3 catalytic subunit beta isoform antibody
    • Protein phosphatase 3 catalytic subunit beta isozyme antibody
    • Serine/threonine protein phosphatase 2B catalytic subunit beta isoform antibody
    • Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform antibody
    see all

Images

  • Immunohistochemical analysis of formalin fixed paraffin embedded human brain cortex labeling PPP3CB with ab191374 at 7.5 µg/ml

  • Anti-PPP3CB antibody (ab191374) at 1/5000 dilution + Molt-4 cell lysate at 30 µg

    Predicted band size: 59 kDa



    7.5 % SDS PAGE

  • Anti-PPP3CB antibody (ab191374) at 1/1000 dilution + Mouse brain lysate at 50 µg

    Predicted band size: 59 kDa



    7.5% SDS PAGE

References

This product has been referenced in:

  • Wang C  et al. Small-molecule TFEB pathway agonists that ameliorate metabolic syndrome in mice and extend C. elegans lifespan. Nat Commun 8:2270 (2017). Read more (PubMed: 29273768) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab191374.
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