Overview

  • Product name

  • Description

    Rabbit polyclonal to PPP4R2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Horse, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 370 and the C-terminus (residue 417) of Human PPP4R2 (NP_777567.1)

  • Positive control

    • Whole cell lysates from HeLa, 293T and NIH3T3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab70631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 47 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Images

    • All lanes : Anti-PPP4R2 antibody (ab70631) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 47 kDa
      Observed band size: 65 kDa
      why is the actual band size different from the predicted?


      Exposure time: 30 seconds
    • Detection of Human PPP4R2 by Western Blot of Immunprecipitate. ab70631 at 1µg/ml staining PPP4R2 in HeLa whole cell lysate immunoprecipitated using ab70631 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: chemiluminescence with exposure time of 10 seconds.

    References

    This product has been referenced in:

    • Ho MY  et al. PATZ1 induces PP4R2 to form a negative feedback loop on IKK/NF-?B signaling in lung cancer. Oncotarget 7:52255-52269 (2016). Read more (PubMed: 27391343) »
    • Chen GI  et al. PP4R4/KIAA1622 forms a novel stable cytosolic complex with phosphoprotein phosphatase 4. J Biol Chem : (2008). Read more (PubMed: 18715871) »
    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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