Key features and details
- Rabbit polyclonal to PPP4R2
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PPP4R2 antibody
See all PPP4R2 primary antibodies
DescriptionRabbit polyclonal to PPP4R2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Horse, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residue 370 and the C-terminus (residue 417) of Human PPP4R2 (NP_777567.1)
- Whole cell lysates from HeLa, 293T and NIH3T3 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70631 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 47 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- Information by UniProt
- BE691708 antibody
- C230060M08Rik antibody
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All lanes : Anti-PPP4R2 antibody (ab70631) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 47 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Detection of Human PPP4R2 by Western Blot of Immunprecipitate. ab70631 at 1µg/ml staining PPP4R2 in HeLa whole cell lysate immunoprecipitated using ab70631 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: chemiluminescence with exposure time of 10 seconds.
ab70631 has been referenced in 2 publications.