Anti-PPP4R2 antibody (ab70631)
Key features and details
- Rabbit polyclonal to PPP4R2
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PPP4R2 antibody -
Description
Rabbit polyclonal to PPP4R2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Horse, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to a region between residue 370 and the C-terminus (residue 417) of Human PPP4R2 (NP_777567.1)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70631 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200.
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WB |
1/2000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 47 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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IHC-P
1/200. |
WB
1/2000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 47 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 151987 Human
- Entrez Gene: 232314 Mouse
- Entrez Gene: 100172791 Orangutan
- Omim: 613822 Human
- SwissProt: Q9NY27 Human
- SwissProt: Q0VGB7 Mouse
- SwissProt: Q5R9U6 Orangutan
- Unigene: 431092 Human
see all -
Alternative names
- BE691708 antibody
- C230060M08Rik antibody
- MGC131930 antibody
see all
Images
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All lanes : Anti-PPP4R2 antibody (ab70631) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 47 kDa -
Immunohistochemical analysis of human ovarian cancer tissue, labelling PPP4R2 with ab70631 at 1/200 dilution.
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Detection of Human PPP4R2 by Western Blot of Immunprecipitate. ab70631 at 1µg/ml staining PPP4R2 in HeLa whole cell lysate immunoprecipitated using ab70631 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: chemiluminescence with exposure time of 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab70631 has been referenced in 2 publications.
- Ho MY et al. PATZ1 induces PP4R2 to form a negative feedback loop on IKK/NF-?B signaling in lung cancer. Oncotarget 7:52255-52269 (2016). PubMed: 27391343
- Chen GI et al. PP4R4/KIAA1622 forms a novel stable cytosolic complex with phosphoprotein phosphatase 4. J Biol Chem : (2008). PubMed: 18715871