Product nameAnti-PPP4R2 antibody
See all PPP4R2 primary antibodies
DescriptionRabbit polyclonal to PPP4R2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Horse, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residue 370 and the C-terminus (residue 417) of Human PPP4R2 (NP_777567.1)
- Whole cell lysates from HeLa, 293T and NIH3T3 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70631 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 47 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- Information by UniProt
- BE691708 antibody
- C230060M08Rik antibody
- MGC131930 antibody
All lanes : Anti-PPP4R2 antibody (ab70631) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 47 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Detection of Human PPP4R2 by Western Blot of Immunprecipitate. ab70631 at 1µg/ml staining PPP4R2 in HeLa whole cell lysate immunoprecipitated using ab70631 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: chemiluminescence with exposure time of 10 seconds.
This product has been referenced in:
- Ho MY et al. PATZ1 induces PP4R2 to form a negative feedback loop on IKK/NF-?B signaling in lung cancer. Oncotarget 7:52255-52269 (2016). Read more (PubMed: 27391343) »
- Chen GI et al. PP4R4/KIAA1622 forms a novel stable cytosolic complex with phosphoprotein phosphatase 4. J Biol Chem : (2008). Read more (PubMed: 18715871) »