Key features and details
- Rabbit polyclonal to PPP6C/Ppv
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PPP6C/Ppv antibody
See all PPP6C/Ppv primary antibodies
DescriptionRabbit polyclonal to PPP6C/Ppv
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
Synthetic peptide corresponding to Human PPP6C/Ppv (C terminal).
Database link: NP_002712.1
- Whole cell lysates from HeLa, 293T and NIH3T3 cells.
This product was previously labelled as PPP6C
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.764% Sodium citrate, 1.815% Tris, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70634 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionCatalytic subunit of protein phospatase 6 (PP6). PP6 is a component of a signaling pathway regulating cell cycle progression in response to IL2 receptor stimulation. N-terminal domain restricts G1 to S phase progression in cancer cells, in part through control of cyclin D1. Downregulates MAP3K7 kinase activation of the IL1 signaling pathway by dephosphorylation of MAP3K7.
Tissue specificityUbiquitously expressed in all tissues tested with highest expression levels in testis, heart, kidney, brain, stomach, liver and skeletal muscle and lowest in placenta, lung colon and spleen.
Sequence similaritiesBelongs to the PPP phosphatase family. PP-6 (PP-V) subfamily.
- Information by UniProt
- FLJ92648 antibody
- MGC12249 antibody
- PP 6 antibody
All lanes : Anti-PPP6C/Ppv antibody (ab70634) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 35 kDa
Observed band size: 35 kDa
Exposure time: 3 minutes
Detection of PPP6C/Ppv by Western Blot of Immunoprecipitate. ab70634 at 1µg/ml staining PPP6C/Ppv in HeLa whole cell lysate immunoprecipitated using ab70634 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: chemiluminescence with exposure time of 30 seconds.
ab70634 has been referenced in 1 publication.
- Gallegos LL et al. A protein interaction map for cell-cell adhesion regulators identifies DUSP23 as a novel phosphatase for ß-catenin. Sci Rep 6:27114 (2016). PubMed: 27255161