Anti-PPP6C/Ppv antibody (ab70634)
Key features and details
- Rabbit polyclonal to PPP6C/Ppv
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PPP6C/Ppv antibody
See all PPP6C/Ppv primary antibodies -
Description
Rabbit polyclonal to PPP6C/Ppv -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis -
Immunogen
Synthetic peptide corresponding to Human PPP6C/Ppv (C terminal).
Database link: NP_002712.1 -
Positive control
- Whole cell lysates from HeLa, 293T and NIH3T3 cells.
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General notes
This product was previously labelled as PPP6C
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.764% Sodium citrate, 1.815% Tris, 0.021% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab70634 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). | |
IP | Use at 2-5 µg/mg of lysate. |
Target
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Function
Catalytic subunit of protein phospatase 6 (PP6). PP6 is a component of a signaling pathway regulating cell cycle progression in response to IL2 receptor stimulation. N-terminal domain restricts G1 to S phase progression in cancer cells, in part through control of cyclin D1. Downregulates MAP3K7 kinase activation of the IL1 signaling pathway by dephosphorylation of MAP3K7. -
Tissue specificity
Ubiquitously expressed in all tissues tested with highest expression levels in testis, heart, kidney, brain, stomach, liver and skeletal muscle and lowest in placenta, lung colon and spleen. -
Sequence similarities
Belongs to the PPP phosphatase family. PP-6 (PP-V) subfamily. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 511058 Cow
- Entrez Gene: 608922 Dog
- Entrez Gene: 5537 Human
- Entrez Gene: 67857 Mouse
- Entrez Gene: 100153256 Pig
- Entrez Gene: 171121 Rat
- Entrez Gene: 734439 Xenopus laevis
- Entrez Gene: 393980 Zebrafish
see all -
Alternative names
- FLJ92648 antibody
- MGC12249 antibody
- PP 6 antibody
see all
Images
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All lanes : Anti-PPP6C/Ppv antibody (ab70634) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 35 kDa
Observed band size: 35 kDa
Exposure time: 3 minutes -
Detection of PPP6C/Ppv by Western Blot of Immunoprecipitate. ab70634 at 1µg/ml staining PPP6C/Ppv in HeLa whole cell lysate immunoprecipitated using ab70634 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: chemiluminescence with exposure time of 30 seconds.
Protocols
References (1)
ab70634 has been referenced in 1 publication.
- Gallegos LL et al. A protein interaction map for cell-cell adhesion regulators identifies DUSP23 as a novel phosphatase for ß-catenin. Sci Rep 6:27114 (2016). PubMed: 27255161