Anti-PPP6C/Ppv antibody (ab70634)

Reviews (1)Q&A (6)References (1)
Western blot - Anti-PPP6C/Ppv antibody (ab70634)
  • Immunoprecipitation - Anti-PPP6C/Ppv antibody (ab70634)

Key features and details

  • Rabbit polyclonal to PPP6C/Ppv
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-PPP6C/Ppv antibody
    See all PPP6C/Ppv primary antibodies

  • Description

    Rabbit polyclonal to PPP6C/Ppv

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis

  • Immunogen

    Synthetic peptide corresponding to Human PPP6C/Ppv (C terminal).
    Database link: NP_002712.1

  • Positive control

    • Whole cell lysates from HeLa, 293T and NIH3T3 cells.

  • General notes

     This product was previously labelled as PPP6C

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituents: 1.764% Sodium citrate, 1.815% Tris, 0.021% PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70634 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Catalytic subunit of protein phospatase 6 (PP6). PP6 is a component of a signaling pathway regulating cell cycle progression in response to IL2 receptor stimulation. N-terminal domain restricts G1 to S phase progression in cancer cells, in part through control of cyclin D1. Downregulates MAP3K7 kinase activation of the IL1 signaling pathway by dephosphorylation of MAP3K7.

  • Tissue specificity

    Ubiquitously expressed in all tissues tested with highest expression levels in testis, heart, kidney, brain, stomach, liver and skeletal muscle and lowest in placenta, lung colon and spleen.

  • Sequence similarities

    Belongs to the PPP phosphatase family. PP-6 (PP-V) subfamily.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • FLJ92648 antibody
    • MGC12249 antibody
    • PP 6 antibody
    • PP6 antibody
    • PP6C antibody
    • PPP 6 antibody
    • PPP 6C antibody
    • PPP6 antibody
    • PPP6_HUMAN antibody
    • Ppp6c antibody
    • Protein phosphatase 6 catalytic subunit antibody
    • Serine/threonine protein phosphatase 6 antibody
    • Serine/threonine protein phosphatase catalytic subunit antibody
    • Serine/threonine-protein phosphatase 6 catalytic subunit antibody
    see all

Images

  • Western blot - Anti-PPP6C/Ppv antibody (ab70634)
    Western blot - Anti-PPP6C/Ppv antibody (ab70634)
    All lanes : Anti-PPP6C/Ppv antibody (ab70634) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 35 kDa
    Observed band size: 35 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation - Anti-PPP6C/Ppv antibody (ab70634)
    Immunoprecipitation - Anti-PPP6C/Ppv antibody (ab70634)

    Detection of PPP6C/Ppv by Western Blot of Immunoprecipitate. ab70634 at 1µg/ml staining PPP6C/Ppv in HeLa whole cell lysate immunoprecipitated using ab70634 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: chemiluminescence with exposure time of 30 seconds.

References (1)

Publishing research using ab70634? Please let us know so that we can cite the reference in this datasheet.

ab70634 has been referenced in 1 publication.

  • Gallegos LL  et al. A protein interaction map for cell-cell adhesion regulators identifies DUSP23 as a novel phosphatase for ß-catenin. Sci Rep 6:27114 (2016). PubMed: 27255161

Customer reviews and Q&As

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1-7 of 7 Abreviews or Q&A

Question

Hi:

I would give the other antibody, the https://www.abcam.com/PPP6C-antibody-EPR8764-ab131335.html a go. Thanks!

Read More
Answer

Thank you for confirming that.

I have now raised an order to have the anti-PPP6C antibody (ab131335) to be sent to you free of charge. This is on the order number xxxxx (Purchase order number FOCR xxxxxx). Unfortunately this antibody is currently not in stock but should be with you in no more than 2 weeks. I am sorry for this delay and any inconvenience it may cause.

I hope the result with this antibody will be an improvement on that observed with ab70634. Please do let me know how you get on with it, regardless of if the results are positive or not.

Until then, I wish you all the best with your research.

Read More

Question

Hi:

To answer your questions:

1. The buffer is what I use, the lysis buffer is that with 1% triton (added before the lysis).

2. Yes, it is a very good antibody

3. I probed the sample for another band (a phospho antibody and am probing for loading control now, but the last blot which also has not worked, has been probed for a protein called PAK1 and actin)

4. The Odyssey Licor appratus was used at other power settings as well as highest power.

Thanks

Read More
Answer

Thank you for taking time to provide me with that information. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

Having reviewed the protocol details provided, I believe this product should have given satisfactory results. It unfortunately appears that you may have received a faulty vial.

I am sorry for the inconvenience this has caused you. I am able to offer an alternative vial of ab70634 if you would like (although we do not currently have a different lot to the one you have used). Alternatively I can provide you with an alternative antibody altogether, such as the rabbit monoclonal https://www.abcam.com/PPP6C-antibody-EPR8764-ab131335.html. If you would prefer, I can also provide a credit note, or refund in compensation.

Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

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Question

Hi Attached is the filled in form, please contact for any more info, I would appreciate it if this can be resolved quickly.

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Answer

Thank you for providing that information.

I will try to help as best I can to resolve these problems. The information provided has been very helpful in understanding the experiment you have been performing. I do have a few follow up questions to ask just to be able to ascertain what may be contributing to this lack of signal from the samples.

1. How were the lysates prepared? The buffer composition you shared, was this used as the lysis buffer as well as the SDS-page loading buffer?

2. Has the secondary antibody (goat anti-rabbit Alexa Fluor 680) been used previously with a different primary antibody? How were these results?

3. Was any loading control used with the samples? For example actin or GAPDH probed? What was the results of this?

4. What exposure was used on the membrane?

I thank you for your cooperation and look forward to receiving your reply.

Read More

Question

Phone call reporting problems in using the Anti-PPP6C antibody (ab70634).

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Answer

Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
As we discussed on the phone, I have attached our questionnaire so thatI can gather further information regarding the samples tested and the protocol used. Once I have receive the completed questionnaire,I will look at the protocol and see if there are any suggestions I can make that may improve the results.

This information will also allow us to investigate this case internally and initiate additional testing where necessary.

If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

I look forward to receiving your reply.

Read More

Question

will test either ab70634 or ab60249 in Arabidopsis

Read More
Answer

Thank you for contacting us.

Please let me know which antibody you would like to try inArabidopsis (ab70634 or ab60249), so that I can send you the testing discount code.

I look forward to hear back from you.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

I have a question about an antibody cross-species specificity. I found antibodies against human PP6 in your catalog: ab60249 and ab70634. I am wondering whether these antibodies are able to recognize Drosophila (fruit fly) PP6 protein. Do you have any information about that? Although the PP6 protein is highly conserved between human and fly species, I am still not quite sure whether your antibodies will work or not in fly system. Therefore, I want to get/purchase a small size sample to test in fly. Is it possible? Thank you in advance for your help.

Read More
Answer

Thank you for contacting Abcam. I have confirmed with the laboratory that neither ab60249 nor ab70634 share significant homology with the Drosophila sequence.  We do not expect these antibodies to cross react with this species. I apologize for any inconvenience this may cause.  Please do not hesitate to contact us if you have any additional questions.

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Western blot abreview for Anti-PPP6C antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (fibroblast)
Loading amount
25 µg
Specification
fibroblast
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Verified customer

Submitted Aug 07 2009

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