Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8764] to PPP6C/Ppv
- Suitable for: WB, IP, Flow Cyt, ICC/IF
- Reacts with: Mouse, Rat, Human
Product nameAnti-PPP6C/Ppv antibody [EPR8764]
See all PPP6C/Ppv primary antibodies
DescriptionRabbit monoclonal [EPR8764] to PPP6C/Ppv
Tested applicationsSuitable for: WB, IP, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse, Rat, Human
corresponding to Human PPP6C/Ppv aa 250-350 (C terminal).
Database link: O00743
- WB: HeLa, fetal thymus and 293T and Jurkat whole cell lysate (ab7899). ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
This product was previously labelled as PPP6C
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 5.00 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab131335 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 35 kDa.|
|IP||1/10 - 1/100.|
|ICC/IF||1/100 - 1/250.|
FunctionCatalytic subunit of protein phospatase 6 (PP6). PP6 is a component of a signaling pathway regulating cell cycle progression in response to IL2 receptor stimulation. N-terminal domain restricts G1 to S phase progression in cancer cells, in part through control of cyclin D1. Downregulates MAP3K7 kinase activation of the IL1 signaling pathway by dephosphorylation of MAP3K7.
Tissue specificityUbiquitously expressed in all tissues tested with highest expression levels in testis, heart, kidney, brain, stomach, liver and skeletal muscle and lowest in placenta, lung colon and spleen.
Sequence similaritiesBelongs to the PPP phosphatase family. PP-6 (PP-V) subfamily.
- Information by UniProt
- FLJ92648 antibody
- MGC12249 antibody
- PP 6 antibody
All lanes : Anti-PPP6C/Ppv antibody [EPR8764] (ab131335) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Fetal thymus lysate
Lane 3 : 293T cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 35 kDa
Immunofluorescent analysis of HeLa cells labelling PPP6C/Ppv with ab131335 at 1/100 dilution.
Flow Cytometry analysis of HeLa cells labelling PPP6C/Ppv with purified ab131335 at a dilution of 1/280 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Flour® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab131335 has been referenced in 3 publications.
- Moffett HF et al. The microRNA miR-31 inhibits CD8+ T cell function in chronic viral infection. Nat Immunol 18:791-799 (2017). WB ; Mouse . PubMed: 28530712
- Yan S et al. NF-?B-induced microRNA-31 promotes epidermal hyperplasia by repressing protein phosphatase 6 in psoriasis. Nat Commun 6:7652 (2015). PubMed: 26138368
- Montoya V et al. Novel miRNA-31 and miRNA-200a-Mediated Regulation of Retinoblastoma Proliferation. PLoS One 10:e0138366 (2015). WB, IF . PubMed: 26379276