Overview

  • Product name

    Anti-PPP6C/Ppv antibody [EPR8764]
    See all PPP6C/Ppv primary antibodies
  • Description

    Rabbit monoclonal [EPR8764] to PPP6C/Ppv
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human PPP6C/Ppv aa 250-350 (C terminal).
    Database link: O00743

  • Positive control

    • WB: HeLa, fetal thymus and 293T and Jurkat whole cell lysate (ab7899). ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
  • General notes

     This product was previously labelled as PPP6C

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 5.00 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8764
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab131335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/280.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Catalytic subunit of protein phospatase 6 (PP6). PP6 is a component of a signaling pathway regulating cell cycle progression in response to IL2 receptor stimulation. N-terminal domain restricts G1 to S phase progression in cancer cells, in part through control of cyclin D1. Downregulates MAP3K7 kinase activation of the IL1 signaling pathway by dephosphorylation of MAP3K7.
    • Tissue specificity

      Ubiquitously expressed in all tissues tested with highest expression levels in testis, heart, kidney, brain, stomach, liver and skeletal muscle and lowest in placenta, lung colon and spleen.
    • Sequence similarities

      Belongs to the PPP phosphatase family. PP-6 (PP-V) subfamily.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • FLJ92648 antibody
      • MGC12249 antibody
      • PP 6 antibody
      • PP6 antibody
      • PP6C antibody
      • PPP 6 antibody
      • PPP 6C antibody
      • PPP6 antibody
      • PPP6_HUMAN antibody
      • Ppp6c antibody
      • Protein phosphatase 6 catalytic subunit antibody
      • Serine/threonine protein phosphatase 6 antibody
      • Serine/threonine protein phosphatase catalytic subunit antibody
      • Serine/threonine-protein phosphatase 6 catalytic subunit antibody
      see all

    Images

    • All lanes : Anti-PPP6C/Ppv antibody [EPR8764] (ab131335) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Fetal thymus lysate
      Lane 3 : 293T cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 35 kDa

    • Immunofluorescent analysis of HeLa cells labelling PPP6C/Ppv with ab131335 at 1/100 dilution.

    • Flow Cytometry analysis of HeLa cells labelling PPP6C/Ppv with purified ab131335 at a dilution of 1/280 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Flour® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Moffett HF  et al. The microRNA miR-31 inhibits CD8+ T cell function in chronic viral infection. Nat Immunol 18:791-799 (2017). WB ; Mouse . Read more (PubMed: 28530712) »
    • Montoya V  et al. Novel miRNA-31 and miRNA-200a-Mediated Regulation of Retinoblastoma Proliferation. PLoS One 10:e0138366 (2015). WB, IF . Read more (PubMed: 26379276) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Non-reduced Denaturing (18%)
    Sample
    Human Cell lysate - whole cell (293T)
    Specification
    293T
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

    Abcam user community

    Verified customer

    Submitted Aug 27 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up