Western blot abreview for Anti-PPP6C antibody [EPR8764]

Excellent
Abreviews
Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Non-reduced Denaturing (18%)
Sample
Human Cell lysate - whole cell (293T)
Specification
293T
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS tween 5% milk
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL prime
Bands
Specific: 35 kDa Non-specific: 20 kDa
Exposure
1 minute(s) and 0 second(s)

Abcam user community

Verified customer

Submitted Aug 27 2014

Sign up