Overview

  • Product name
  • Description
    Rabbit polyclonal to PPT1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH-conjugated synthetic peptide (10-30 aa in length) at the C-term of last 50 aa of human PPT1.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab38417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 34 kDa.
ELISA 1/1000.
IHC-P 1/50 - 1/100.

Target

  • Function
    Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons.
  • Involvement in disease
    Defects in PPT1 are the cause of neuronal ceroid lipofuscinosis type 1 (CLN1) [MIM:256730]. A form of neuronal ceroid lipofuscinosis with variable age at onset. Infantile, late-infantile, juvenile, and adult onset have been reported. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy. The lipopigment pattern seen most often in CLN1 is referred to as granular osmiophilic deposits (GROD).
  • Sequence similarities
    Belongs to the palmitoyl-protein thioesterase family.
  • Cellular localization
    Lysosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ceroid palmitoyl palmitoyl protein thioesterase 1 antibody
    • CLN1 antibody
    • EC 3.1.2.22 antibody
    • INCL antibody
    • Palmitoyl protein hydrolase 1 antibody
    • Palmitoyl protein thioesterase 1 antibody
    • Palmitoyl-protein hydrolase 1 antibody
    • Palmitoyl-protein thioesterase 1 antibody
    • PPT antibody
    • PPT-1 antibody
    • PPT1 antibody
    • PPT1_HUMAN antibody
    see all

Images

  • Anti-PPT1 antibody (ab38417) at 1/100 dilution + 293 cell lysate.

    Predicted band size: 34 kDa
    Observed band size: 34 kDa

  • Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

References

This product has been referenced in:
  • O'Shaughnessy PJ  et al. Steroidogenic enzyme expression in the human fetal liver and potential role in the endocrinology of pregnancy. Mol Hum Reprod : (2012). Read more (PubMed: 23197595) »
See 1 Publication for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Answer

Thank you for your reply.


As requested, I have issued a free of charge replacement of ab89022.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Answer

Thank you for your reply and for trying out my suggestions.


I think you are making some progress diluting out the secondary antibody. The background is way too high for a secondary antibody and to properly assess the results. I do notice a different band pattern in the THP-1 samples. I would recommend continuing to dilute the secondary (1:70000, 1:100000). I think this will greatly improve your results!


If this does not help, I am happy to offer a replacement. Please keep me informed of your results.

Read More

Answer

Thank you for your reply.


I appreciate that you tested the suggestions made to improve your results. It is very alarming how much background you observe in the absence of primary. As a next step, it is necessary to further dilute the secondary and also consider loading no more than 50ug of protein on the gel. Is it possible that the secondary antibody is contaminated? Do you have another aliquot you can test? It is very difficult to distinguish if the problem is related to one antibody or both following these results.

Read More

Answer

Thank you for your reply.


I would recommend that you also test some higher dilutions of antibody (test 1:250 and 1:500) to help eliminate some background bands. Additionally, I would recommend using 5% BSA rather than milk to help reduce background. If these suggestions do not improve your results, I am happy to offer a replacement per our Abpromise. Please provide an order number with your reply.


I look forward to your reply so that I may assist you further. Please do not hesitate to contact me if you have any additional questions.

Read More

Answer

Thank you for your reply with this information.


The results of your WB with the human liver tissue are very promising, likely reflective of the band of interest as well as some glycosylated protein. What dilutions of antibody did you test? Also, what was the blocking solution? Incubation times and temperatures? Have you performed a no primary control to confirm that the secondary is not contributing to background?

Read More

Answer

Thank you for contacting Abcam regarding ab38417.

I am sorry that you have been experiencing difficulties with this antibody in WB. In order to better understand the problems you are having in WB I would like to get some additional information regarding your samples and protocol. Can you please provide a detailed summary of your sample preparation and WB conditions? An image of your blot would also be very helpful. Once I have this information I can determine if there are any protocol suggestions that I can make. Alternatively, the antibody may be elibile for replacment or credit per our Abpromise guarantee.


I look forward to your reply so that I may assist you further. Please do not hesitate to contact me if you have any additional questions or concerns.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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