Overview

  • Product name

  • Description

    Rabbit polyclonal to PPT1/PPT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog
  • Immunogen

    Recombinant fragment corresponding to Human PPT1/PPT aa 1-306.
    Database link: P50897

  • Positive control

    • 293T and Raji cells. IHC-P: CL1-0 xenograft, mouse fore brain, rat hind brain, and mouse stomach tissues.
  • General notes

     This product was previously labelled as PPT1

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 34 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons.
  • Involvement in disease

    Defects in PPT1 are the cause of neuronal ceroid lipofuscinosis type 1 (CLN1) [MIM:256730]. A form of neuronal ceroid lipofuscinosis with variable age at onset. Infantile, late-infantile, juvenile, and adult onset have been reported. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy. The lipopigment pattern seen most often in CLN1 is referred to as granular osmiophilic deposits (GROD).
  • Sequence similarities

    Belongs to the palmitoyl-protein thioesterase family.
  • Cellular localization

    Lysosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • Ceroid palmitoyl palmitoyl protein thioesterase 1 antibody
    • CLN1 antibody
    • EC 3.1.2.22 antibody
    • INCL antibody
    • Palmitoyl protein hydrolase 1 antibody
    • Palmitoyl protein thioesterase 1 antibody
    • Palmitoyl-protein hydrolase 1 antibody
    • Palmitoyl-protein thioesterase 1 antibody
    • PPT antibody
    • PPT-1 antibody
    • PPT1 antibody
    • PPT1_HUMAN antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded rat hind brain, labelling PPT1/PPT with ab137578 at 1/500 dilution. EDTA based antigen retrieval pH 8.0 for 15 mins. 

  • Anti-PPT1/PPT antibody (ab137578) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 34 kDa



    10% SDS-PAGE
  • Immunohistochemical analysis of paraffin-embedded mouse fore brain, labelling PPT1/PPT with ab137578 at 1/500 dilution. EDTA based antigen retrieval pH 8.0 for 15 mins. 

  • Immunohistochemical analysis of paraffin-embedded mouse stomach, labelling PPT1/PPT with ab137578 at 1/500 dilution. EDTA based antigen retrieval pH 8.0 for 15 mins. 

  • Immunohistochemical analysis of paraffin-embedded CL1-0 (human) xenograft, labelling PPT1/PPT with ab137578 at 1/500 dilution. EDTA based antigen retrieval pH 8.0 for 15 mins. 

References

ab137578 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab137578.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up