Anti-PPT1/PPT antibody (ab89022)
Key features and details
- Mouse polyclonal to PPT1/PPT
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PPT1/PPT antibody
See all PPT1/PPT primary antibodies -
Description
Mouse polyclonal to PPT1/PPT -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human PPT1/PPT. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: NP_000301.1 -
Positive control
- WB: Human liver tissue lysate, PPT1/PPT transfected HEK-293T cell lysate. IHC-P: Human salivary gland tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | ||
IHC-P |
Notes |
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WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons. -
Involvement in disease
Defects in PPT1 are the cause of neuronal ceroid lipofuscinosis type 1 (CLN1) [MIM:256730]. A form of neuronal ceroid lipofuscinosis with variable age at onset. Infantile, late-infantile, juvenile, and adult onset have been reported. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy. The lipopigment pattern seen most often in CLN1 is referred to as granular osmiophilic deposits (GROD). -
Sequence similarities
Belongs to the palmitoyl-protein thioesterase family. -
Cellular localization
Lysosome. - Information by UniProt
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Database links
- Entrez Gene: 5538 Human
- Omim: 600722 Human
- SwissProt: P50897 Human
- Unigene: 3873 Human
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Alternative names
- Ceroid palmitoyl palmitoyl protein thioesterase 1 antibody
- CLN1 antibody
- EC 3.1.2.22 antibody
see all
Images
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Anti-PPT1/PPT antibody (ab89022) at 1 µg/ml + Human liver tissue lysate at 50 µg
Predicted band size: 34 kDa
Observed band size: 34 kDa -
All lanes : Anti-PPT1/PPT antibody (ab89022) at 1 µg/ml
Lane 1 : PPT1/PPT transfected HEK-293T cell lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 34 kDa
Observed band size: 34 kDa -
ab89022 at 3µg/ml staining PPT1/PPT in Human salivary gland by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab89022 has been referenced in 3 publications.
- Sima N et al. Neural stem cells for disease modeling and evaluation of therapeutics for infantile (CLN1/PPT1) and late infantile (CLN2/TPP1) neuronal ceroid lipofuscinoses. Orphanet J Rare Dis 13:54 (2018). WB . PubMed: 29631617
- Shyng C et al. Widespread Expression of a Membrane-Tethered Version of the Soluble Lysosomal Enzyme Palmitoyl Protein Thioesterase-1. JIMD Rep 36:85-92 (2017). PubMed: 28213849
- Wang R et al. Identification of palmitoyl protein thioesterase 1 in human THP1 monocytes and macrophages and characterization of unique biochemical activities for this enzyme. Biochemistry 52:7559-74 (2013). PubMed: 24083319