Product nameAnti-PPWD1 antibody [EPR7439]
See all PPWD1 primary antibodies
DescriptionRabbit monoclonal [EPR7439] to PPWD1
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human PPWD1 aa 1-100. The exact sequence is proprietary.
- HeLa, NIH3T3, and 293T cell lysates, HeLa cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab150356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionPutative peptidylprolyl isomerase (PPIase). PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be involved in pre-mRNA splicing.
Sequence similaritiesBelongs to the cyclophilin-type PPIase family. PPIL1 subfamily.
Contains 1 PPIase cyclophilin-type domain.
Contains 4 WD repeats.
Cellular localizationNucleus. Associated with spliceosomal complexes.
- Information by UniProt
- 4632422M10Rik antibody
- A330090G21Rik antibody
- KIAA0073 antibody
All lanes : Anti-PPWD1 antibody [EPR7439] (ab150356) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : NIH3T3 cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 74 kDa
Observed band size: 74 kDa
Flow Cytometrical analysis of permeabilized HeLa cells with ab150356 antibody at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).
ab150356 has not yet been referenced specifically in any publications.