PR 619, DUB inhibitor (ab144641)
Key features and details
- Cell-permeable, non-selective and reversible DUB inhibitor
- CAS Number: 2645-32-1
- Purity: > 99%
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
PR 619, DUB inhibitor -
Description
Cell-permeable, non-selective and reversible DUB inhibitor -
Biological description
Cell-permeable, non-selective and reversible DUB inhibitor (IC50 values are 5 to 20 μM). Induces polyubiquitinated protein accumulation in cells without directly affecting proteasome activity. Activates autophagy.
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Purity
> 99% -
CAS Number
2645-32-1 -
Chemical structure
Properties
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Chemical name
Thiocyanic acid 2,6-diamino-3,5-pyridinediyl ester -
Molecular weight
223.27 -
Molecular formula
C7H5N5S2 -
PubChem identifier
2817763 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
C1=C(C(=NC(=C1SC#N)N)N)SC#N -
Source
Synthetic
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Research areas
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (9)
ab144641 has been referenced in 9 publications.
- Karapurkar JK et al. CRISPR/Cas9-based genome-wide screening of the deubiquitinase subfamily identifies USP3 as a protein stabilizer of REST blocking neuronal differentiation and promotes neuroblastoma tumorigenesis. J Exp Clin Cancer Res 42:121 (2023). PubMed: 37170124
- Tyagi A et al. CRISPR/Cas9-based genome-wide screening for deubiquitinase subfamily identifies USP1 regulating MAST1-driven cisplatin-resistance in cancer cells. Theranostics 12:5949-5970 (2022). PubMed: 35966591
- Cao WX et al. Precise Temporal Regulation of Post-transcriptional Repressors Is Required for an Orderly Drosophila Maternal-to-Zygotic Transition. Cell Rep 31:107783 (2020). PubMed: 32579915
- Das S et al. Genome-scale screening of deubiquitinase subfamily identifies USP3 as a stabilizer of Cdc25A regulating cell cycle in cancer. Cell Death Differ N/A:N/A (2020). PubMed: 32415280
- Ohsugi T et al. Anti-apoptotic effect by the suppression of IRF1 as a downstream of Wnt/ß-catenin signaling in colorectal cancer cells. Oncogene 38:6051-6064 (2019). PubMed: 31292489
- Skelly MJ et al. Dynamic ubiquitination determines transcriptional activity of the plant immune coactivator NPR1. Elife 8:N/A (2019). PubMed: 31589140
- Tsuchiya H et al. Ub-ProT reveals global length and composition of protein ubiquitylation in cells. Nat Commun 9:524 (2018). PubMed: 29410401
- Ashkenazi A et al. Polyglutamine tracts regulate beclin 1-dependent autophagy. Nature 545:108-111 (2017). PubMed: 28445460
- Inano S et al. RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol Cell 66:622-634.e8 (2017). PubMed: 28575658