Product nameAnti-PR3 antibody [EPR6277]
See all PR3 primary antibodies
DescriptionRabbit monoclonal [EPR6277] to PR3
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP
Species reactivityReacts with: Human
A synthetic peptide corresponding to residues in Human PR3 (UniProt ID: P24158).
- HL60 and Human spleen lysates; Human kidney tissue
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 5.30 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133613 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 27 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionPolymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters.
Sequence similaritiesBelongs to the peptidase S1 family. Elastase subfamily.
Contains 1 peptidase S1 domain.
- Information by UniProt
- ACPA antibody
- AGP 7 antibody
- AGP7 antibody
All lanes : Anti-PR3 antibody [EPR6277] (ab133613) at 1/1000 dilution
Lane 1 : HL60 lysates
Lane 2 : Human spleen lysates
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 27 kDa
Immunohistochemical analysis of PR3 in paraffin embedded Human kidney tissue labelled with ab133613 at a 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab133613 has not yet been referenced specifically in any publications.