Key features and details
- Mouse monoclonal [PR3G-2] to PR3 (FITC)
- Suitable for: WB, IHC-Fr, Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Product nameAnti-PR3 antibody [PR3G-2] (FITC)
See all PR3 primary antibodies
DescriptionMouse monoclonal [PR3G-2] to PR3 (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
SpecificityThis antibody does not inhibit the biological activity of PR3.
Tested applicationsSuitable for: WB, IHC-Fr, Flow Cytmore details
Species reactivityReacts with: Human
Tissue/ cell preparation (Human): a crude granule extract.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab65255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/50. Predicted molecular weight: 28 kDa.|
|Flow Cyt||Use 0.5µg for 106 cells.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionPolymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters.
Sequence similaritiesBelongs to the peptidase S1 family. Elastase subfamily.
Contains 1 peptidase S1 domain.
- Information by UniProt
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Overlay histogram showing THP1 cells stained with ab65255 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab65255, 0.5µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [MOPC-21] (FITC) (ab18435, 0.5µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
ab65255 has been referenced in 1 publication.
- Liu H et al. Proteinase 3 expression on the neutrophils of patients with paroxysmal nocturnal hemoglobinuria. Exp Ther Med 15:2525-2532 (2018). PubMed: 29467851