Overview

  • Product name

    Anti-PR3 antibody [PR3G-2] (FITC)
    See all PR3 primary antibodies
  • Description

    Mouse monoclonal [PR3G-2] to PR3 (FITC)
  • Host species

    Mouse
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Specificity

    This antibody does not inhibit the biological activity of PR3.
  • Tested applications

    Suitable for: WB, IHC-Fr, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation (Human): a crude granule extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab65255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50. Predicted molecular weight: 28 kDa.
IHC-Fr 1/50.
Flow Cyt Use 0.5µg for 106 cells.

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters.
  • Sequence similarities

    Belongs to the peptidase S1 family. Elastase subfamily.
    Contains 1 peptidase S1 domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACPA antibody
    • AGP 7 antibody
    • AGP7 antibody
    • AGP7 serine proteinase antibody
    • Azurophil Granule Protein 7 antibody
    • C ANCA antibody
    • C ANCA antigen antibody
    • C-ANCA antigen antibody
    • CANCA antibody
    • EC 3.4.21.76 antibody
    • Leukocyte proteinase 3 antibody
    • MBN antibody
    • MBT antibody
    • MBT WEGENER AUTOANTIGEN antibody
    • Myeloblastin antibody
    • Neutrophil proteinase 4 antibody
    • NP 4 antibody
    • NP-4 antibody
    • NP4 antibody
    • P29 antibody
    • PR 3 antibody
    • PR-3 antibody
    • PR3 antibody
    • Proteinase 3 antibody
    • Proteinase3 antibody
    • PRTN 3 antibody
    • Prtn3 antibody
    • PRTN3_HUMAN antibody
    • Serine proteinase neutrophil Wegener granulomatosis autoantigen antibody
    • Serine proteinase, neutrophil antibody
    • Wegener autoantigen antibody
    • Wegener granulomatosis autoantigen antibody
    see all

Images

  • Overlay histogram showing THP1 cells stained with ab65255 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab65255, 0.5µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [MOPC-21] (FITC) (ab18435, 0.5µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

References

This product has been referenced in:

  • Liu H  et al. Proteinase 3 expression on the neutrophils of patients with paroxysmal nocturnal hemoglobinuria. Exp Ther Med 15:2525-2532 (2018). Read more (PubMed: 29467851) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for contacting us.

The protein does express inperipheral blood lymphocytes and neutrophils.

http://www.uniprot.org/uniprot/P24158

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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