Product namePR3 overexpression 293T lysate (whole cell)
DescriptionPR3 overexpression lysate
ab94310 is a 293T cell transfected lysate in which Human PR3 has been transiently over-expressed using a pCMV-PR3 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 6.80
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol
Concentration information loading...
BackgroundFunction: Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters. Similarity: Belongs to the peptidase S1 family. Elastase subfamily. Contains 1 peptidase S1 domain.
Our Abpromise guarantee covers the use of ab94310 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94310 at 15μg/lane on an SDS-PAGE gel.
All lanes : Anti-PR3 antibody (ab88097) at 1/500 dilution
Lane 1 : PR3 293T Transfected Lysate - Positive Control (ab94310)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugate at 1/2500 dilution
ab94310 has not yet been referenced specifically in any publications.