Overview

  • Product name

    Anti-PRA1 antibody [EPR1747Y]
    See all PRA1 primary antibodies
  • Description

    Rabbit monoclonal [EPR1747Y] to PRA1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PRA1 (C terminal). The exact sequence is proprietary.

  • Positive control

    • 293 cell lysate
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab76413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/20000. Predicted molecular weight: 21 kDa.
IP 1/30.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Function

      General Rab protein regulator required for vesicle formation from the Golgi complex. May control vesicle docking and fusion by mediating the action of Rab GTPases to the SNARE complexes. In addition it inhibits the removal of Rab GTPases from the membrane by GDI.
    • Tissue specificity

      Ubiquitous. Strongest expression found in placenta, pituitary gland, kidney, lung and stomach.
    • Sequence similarities

      Belongs to the PRA1 family.
    • Developmental stage

      In fetal tissues, it is more abundant in kidney and lung.
    • Cellular localization

      Cell membrane. Cytoplasm. Golgi apparatus. Cytoplasmic vesicle > secretory vesicle > synaptic vesicle. According to some authors, it is an integral membrane protein, while others showed that it is cytoplasmic and membrane-associated to Golgi and synaptic vesicles.
    • Information by UniProt
    • Database links

    • Alternative names

      • PRA1 domain family 1 antibody
      • PRA1 family protein 1 antibody
      • PRAF1 antibody
      • PRAF1_HUMAN antibody
      • prenylated Rab acceptor 1 antibody
      • Prenylated Rab acceptor protein 1 antibody
      • Rab acceptor 1 (prenylated) antibody
      • RABAC1 antibody
      • YIP3 antibody
      see all

    Images

    • Anti-PRA1 antibody [EPR1747Y] (ab76413) at 1/2000 dilution + 293 cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 21 kDa
      Observed band size: 21 kDa

    References

    This product has been referenced in:

    • Xiao Y  et al. Inhibition of cell proliferation and tumor growth of colorectal cancer by inhibitors of Wnt and Notch signaling pathways. Oncol Lett 12:3695-3700 (2016). WB . Read more (PubMed: 27900056) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Answer

    Thank you for contacting us. The concentration for ab76413, lot GR28817-2, is 1.095 mg/mL. It was measured using ELISA, so the IgG concentration is accurate for a tissue culture supernatant. We do not measure concentration with a UV spectrophotometer in this case. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up