Key features and details
- Rabbit polyclonal to PRA1 - N-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PRA1 antibody - N-terminal
See all PRA1 primary antibodies
DescriptionRabbit polyclonal to PRA1 - N-terminal
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig
- HEK-293T cell lysate overexpressing Human PRA1; HUVEC cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 99% PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab213569 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 21 kDa.|
FunctionGeneral Rab protein regulator required for vesicle formation from the Golgi complex. May control vesicle docking and fusion by mediating the action of Rab GTPases to the SNARE complexes. In addition it inhibits the removal of Rab GTPases from the membrane by GDI.
Tissue specificityUbiquitous. Strongest expression found in placenta, pituitary gland, kidney, lung and stomach.
Sequence similaritiesBelongs to the PRA1 family.
Developmental stageIn fetal tissues, it is more abundant in kidney and lung.
Cellular localizationCell membrane. Cytoplasm. Golgi apparatus. Cytoplasmic vesicle > secretory vesicle > synaptic vesicle. According to some authors, it is an integral membrane protein, while others showed that it is cytoplasmic and membrane-associated to Golgi and synaptic vesicles.
- Information by UniProt
- PRA1 domain family 1 antibody
- PRA1 family protein 1 antibody
- PRAF1 antibody
Lanes 1-2 : Anti-PRA1 antibody - N-terminal (ab213569) at 1/500 dilution
Lane 3 : Anti-PRA1 antibody - N-terminal (ab213569) at 1/1000 dilution
Lanes 1 & 3 : HEK-293T cell lysate overexpressing Human PRA1
Lane 2 : HEK-293T cell lysate overexpressing Human PRA1 with PRA1 immunizing peptide (Human, 2-15)
All lanes : Goat Anti-Rabbit IgG-Peroxidase
Developed using the ECL technique.
Predicted band size: 21 kDa
Immunofluorescent analysis of HUVEC cells labeling PRA1 with ab213569 at 1/50 dilution (red). Nuclear staining with Hoescht 33342 (blue).
ab213569 has not yet been referenced specifically in any publications.