Key features and details
- Rabbit polyclonal to PRAF1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PRAF1 antibody
See all PRAF1 primary antibodies
DescriptionRabbit polyclonal to PRAF1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Sheep, Rabbit, Horse, Cow, Cat, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Common marmoset, Orangutan, Elephant
- HeLa, 293T, and Jurkat whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab206686 was affinity purified using an epitope specific to PRAF1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab206686 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 54 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF.
Sequence similaritiesBelongs to the eukaryotic RPA49/POLR1E RNA polymerase subunit family.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- A49 antibody
- DNA directed RNA polymerase I subunit RPA49 antibody
- DNA-directed RNA polymerase I subunit E antibody
Detection of PRAF1 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab206686 at 6 µg/mg lysate for IP and 1µg/ml for WB detection (Lane 1). Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
All lanes : Anti-PRAF1 antibody (ab206686) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 54 kDa
Exposure time: 3 minutes
ab206686 has not yet been referenced specifically in any publications.