Overview

  • Product name

  • Description

    Rabbit polyclonal to PRAF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Sheep, Rabbit, Horse, Cow, Cat, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Common marmoset, Orangutan, Elephant
  • Immunogen

    Synthetic peptide within Human PRAF1 aa 225-275. The exact sequence is proprietary. Q9GZS1.2.
    Sequence:

    SLNRAV AKAAETIIDT KGVTALVSDA IHNDLQDDSL YLPPCYDDAA KPEDV


    Database link: Q9GZS1

  • Positive control

    • HeLa, 293T, and Jurkat whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab206686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 54 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF.
  • Sequence similarities

    Belongs to the eukaryotic RPA49/POLR1E RNA polymerase subunit family.
  • Cellular localization

    Nucleus > nucleolus.
  • Information by UniProt
  • Database links

  • Alternative names

    • A49 antibody
    • DNA directed RNA polymerase I subunit RPA49 antibody
    • DNA-directed RNA polymerase I subunit E antibody
    • DNA-directed RNA polymerase I subunit RPA49 antibody
    • FLJ 13390 antibody
    • FLJ13970 antibody
    • PAF53 antibody
    • POLR 1 E antibody
    • POLR1E antibody
    • Polymerase (RNA) I associated factor 1 antibody
    • Polymerase (RNA) I polypeptide E antibody
    • Polymerase (RNA) I polypeptide E, 53kDa antibody
    • PRAF1 antibody
    • RNA polymerase I associated factor 1 antibody
    • RNA polymerase I associated factor 53 antibody
    • RNA polymerase I polypeptide E antibody
    • RNA polymerase I subunit A49 antibody
    • RNA polymerase I-associated factor 1 antibody
    • RNA polymerase I-associated factor 53 antibody
    • RP11405L18.3 antibody
    • RPA49_HUMAN antibody
    see all

Images

  • Detection of PRAF1 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab206686 at 6 µg/mg lysate for IP and 1µg/ml for WB detection (Lane 1). Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.

  • All lanes : Anti-PRAF1 antibody (ab206686) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T
    Lane 3 : Jurkat whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 54 kDa


    Exposure time: 3 minutes

References

ab206686 has not yet been referenced specifically in any publications.

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