Key features and details
- Rabbit polyclonal to PRAGMIN/SGK223
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PRAGMIN/SGK223 antibody
See all PRAGMIN/SGK223 primary antibodies
DescriptionRabbit polyclonal to PRAGMIN/SGK223
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
- IHC-P: Human tonsil, duodenum, thyroid gland, fallopian tube and liver tissues. WB: RT4 and U-251 MG cell lysates. ICC/IF: U-251 MG cells.
This product was previously labelled as PRAGMIN
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab246903 in the following tested applications.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 149 kDa.|
Sequence similaritiesBelongs to the protein kinase superfamily.
Contains 1 protein kinase domain.
- Information by UniProt
- D8Ertd82e antibody
- DKFZp761P0423 antibody
- DNA segment, Chr 8, ERATO Doi 82 antibody
PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for PRAGMIN/SGK223 (green) using ab246903 at 4 µg/ml in ICC/IF.
All lanes : Anti-PRAGMIN/SGK223 antibody (ab246903) at 0.4 µg/ml
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) cell lysate
Predicted band size: 149 kDa
Paraffin-embedded human thyroid gland tissue stained for PRAGMIN/SGK223 using ab246903 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human duodenum tissue stained for PRAGMIN/SGK223 using ab246903 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human tonsil tissue stained for PRAGMIN/SGK223 using ab246903 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human fallopian tube tissue stained for PRAGMIN/SGK223 using ab246903 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human liver tissue stained for PRAGMIN/SGK223 using ab246903 at 1/200 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab246903 has not yet been referenced specifically in any publications.